1887

Abstract

Abstract

Most probable numbers for bacteria degrading stearate were 1.5 x 10 to 4.6 x 10, 2.4 × 10, and 4.6 × 10 cells per ml of municipal anaerobic digester sludge, swine waste lagoon sediment, and cow ruminai fluid, respectively. Strain SD2, which was isolated from digestor sludge in coculture with the H-utilizing bacterium sp. strain G-11, plus sulfate, was a long-chain saturated fatty-acid-using bacterium identified as a strain of the genus Strain SD2 differed from subsp. subsp. nov. in its usually smaller size and in its ability to catabolize C to C saturated fatty acids. It differed from in not needing relatively high concentrations of Ca and in its inability to catabolize unsaturated fatty acids, such as oleic or linoleic acid. A strain SD2 coculture was further adapted to grow on crotonate and was subsequently purified without sulfate, with cells of strain G-11 only rarely seen. A 16S ribosomal ribonucleic acid sequence analysis (H. Zhao, D. Yang, C. Woese, and M. P. Bryant, manuscript in preparation) indicated that strain SD2 is phylogenetically very closely related to Thus, we propose the name subsp. for this organism (strain DSM 4212) (T = type strain) because of its ecologically important ability to use stearate and other long-chain saturated fatty acids. Emended descriptions of the genus and species are given.

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1989-04-01
2024-03-28
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References

  1. Balch W. E., Wolfe R. S. 1976; New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl. Environ. Microbiol. 32:781–791
    [Google Scholar]
  2. Balch W. E., Wolfe R. S. 1979; Specificity and biological distribution of coenzyme M (2-mercaptoethanesulfonic acid). J. Bacteriol. 137:256–263
    [Google Scholar]
  3. Barker H. A. 1936; On the fermentation of some dibasic C4 acids by Aerobacter aerogenes. K. Akad. Wet. Amsterdam Proc. 39:674–783
    [Google Scholar]
  4. Beaty P. S., Mclnerney M. J. 1987; Growth of Syntrophomonas wolfei in pure culture on crotonate. Arch. Microbiol. 147:389–393
    [Google Scholar]
  5. Bryant M. P. 1972; Commentary on the Hungate technique for culture of anaerobic bacteria. Am. J. Clin. Nutr. 25:1324–1328
    [Google Scholar]
  6. Chynoweth D. P., Mah R. A. 1971; Volatile acid formation in sludge digestion. Adv. Chem. Ser. 105:41–54
    [Google Scholar]
  7. Doetsch R. N. 1981; Determinative methods of light microscopy. 29–30 In Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. (ed.) Manual of methods for general microbiology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  8. Fay J. P., Farias R. N. 1981; β-oxidation-mediated resistance of Escherichia coli to inhibition by long-chain fatty acids. Curr. Microbiol. 5:147–152
    [Google Scholar]
  9. Fogo J. Κ., Popowsky M. 1949; Spectrophotometric determination of hydrogen sulfide. Anal. Chem. 21:732–734
    [Google Scholar]
  10. Heukelekian H. 1958; Basic principles of sludge digestion. 25–43 In McCabe J., Echkenfelder W. W. (ed.) Biological treatment of sewage and industrial wastes vol. 2 Anaerobic digestion and solids separation; Reinhold, New York:
    [Google Scholar]
  11. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bacteriol. Rev. 14:1–44
    [Google Scholar]
  12. Jeris J. S., McCarty P. L. 1965; The biochemistry of methane fermentation using 14C tracers. J. Water Pollut. Control Fed. 37:178–192
    [Google Scholar]
  13. McCarty P. L. 1966; Kinetics of waste assimilation in anaerobic treatment. Dev. Ind. Microbiol. 7:144–155
    [Google Scholar]
  14. McCarty P. L. 1971; Energetics and kinetics of anaerobic treatment. Adv. Chem. Ser. 105:91–107
    [Google Scholar]
  15. Mclnerney M. J., Bryant M. P., Hespell R. B., Costerton J. W. 1981; Syntrophomonas wolfei gen. nov., sp. nov., an anaerobic, syntrophic, fatty-acid-oxidizing bacterium. Appl. Environ. Microbiol. 41:1029–1039
    [Google Scholar]
  16. Mclnerney M. J., Bryant M. P., Pfennig N. 1979; Anaerobic bacterium that degrades fatty acids in syntrophic association with methanogens. ArcH. Microbiol. 122:129–135
    [Google Scholar]
  17. Mclnerney M. J., Mackie R. I., Bryant M. P. 1981; Syntrophic association of a butyrate-degrading bacterium and Methanosarcina enriched from bovine rumen fluid. Appl. Environ. Microbiol. 41:826–828
    [Google Scholar]
  18. Roy F., Samain E., Dubourgier H. C., Albagnac G. 1986; Syntrophomonas sapovorans sp. nov., a new obligately proton reducing anaerobe oxidizing saturated and unsaturated long chain fatty acids. Arch. Microbiol. 145:142–147
    [Google Scholar]
  19. Salanitro J. P., Muirhead P. A. 1975; Quantitative method for the gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media. Appl. Microbiol. 29:374–381
    [Google Scholar]
  20. Schink B. 1985; Degradation of unsaturated hydrocarbons by methanogenic enrichment cultures. FEMS Microbiol. Ecol. 31:69–77
    [Google Scholar]
  21. Shelton D. R., Tiedje J. M. 1984; Isolation and partial characterization of bacteria in an anaerobic consortium that mineralizes 3-chlorobenzoic acid. Appl. Environ. Microbiol. 48:840–848
    [Google Scholar]
  22. Stieb M., Schink B. 1985; Anaerobic oxidation of fatty acids by Clostridum bryantii sp. nov., a sporeforming, obligately syntrophic bacterium. Arch. Microbiol. 140:387–390
    [Google Scholar]
  23. Weng C. N., Jeris J. S. 1976; Biochemical mechanisms in the methane fermentation of glutamic and oleic acids. Water Res. 10:9–18
    [Google Scholar]
  24. Widdel F. 1986; Growth of methanogenic bacteria in pure culture with 2-propanol and other alchohols as hydrogen donors. Appl. Environ. Microbiol. 51:1056–1062
    [Google Scholar]
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