1887

Abstract

A novel moderately thermophilic, weakly acidophilic, heterotrophic, anaerobic, short-rod bacterium having an outer sheath-like structure (toga) was isolated from a low-salt acidic terrestrial hot spring in Oku-Shiobara, Tochigi, Japan. The strain, designated NAS-01, grew between 30 and 60 °C (optimum 55 °C), and at pH 4.5 and 7.5 (optimum pH 5.5 to 6.0) and could not grow in media with ≥ 1 % NaCl (optimum 0 % NaCl). It utilized Fe(III), thiosulfate or -cystine as electron acceptor for growth, and yeast extract, peptone or a variety of sugars as carbon and energy sources. The major cellular fatty acid was C, and no lipoquinone was detected. Strain NAS-01 contained phospholipids and glycolipids, but not aminolipids. The DNA G+C content was 41.1 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain was included in the phylum , and was closely related to . The 16S rRNA gene sequence similarity between them was 90 %, and they were deeply branched off the rest of the known species. On the basis of the phylogenetic and ecophysiological properties, strain NAS-01 represents a novel species of a new genus in the phylum , for which we propose the name gen. nov., sp. nov. The type strain of the type species is NAS-01 ( = JCM 19762 = DSM 28954). In addition, we propose a new order and family, ord. nov. and fam. nov., respectively, to accommodate the novel genus and the closely related genus .

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2016-02-01
2024-03-29
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