| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
E-mail contact: tokano{at}abmes.twmu.ac.jp
Recently, the field of tissue engineering has progressed rapidly, but poor vascularization remains a major obstacle in bioengineering cell-dense tissues, limiting the viable size of constructs due to hypoxia, nutrient insufficiency, and waste accumulation. Therefore, new technologies for fabricating functional tissues with a well-organized vasculature are required. In the present study, neonatal rat cardiomyocytes were harvested as intact sheets from temperature-responsive culture dishes and stacked into cell-dense myocardial tissues. However, the thickness limit for layered cell sheets in subcutaneous tissue was ~80 μm (3 layers). To overcome this limitation, repeated transplantation of triple-layer grafts was performed at 1, 2, or 3 day intervals. The two overlaid grafts completely synchronized and the whole tissues survived without necrosis in the 1 or 2 day interval cases. Multistep transplantation also created ~1 mm thick myocardium with a well-organized microvascular network. Furthermore, functional multilayer grafts fabricated over a surgically connectable artery and vein revealed complete graft perfusion via the vessels and ectopic transplantation of the grafts was successfully performed using direct vessel anastomoses. These cultured cell sheet integration methods overcome long-standing barriers to producing thick, vascularized tissues, revealing a possible solution for the clinical repair of various damaged organs, including the impaired myocardium.
Key words: poly(N-isopropylacrylamide) • electrophysiology • microsurgery
This article has been cited by other articles:
|
|
 |
|
  P. Akhyari, H. Kamiya, A. Haverich, M. Karck, and A. Lichtenberg Myocardial tissue engineering: the extracellular matrix. Eur. J. Cardiothorac. Surg., August 1, 2008; 34(2): 229 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Siepe, P. Akhyari, A. Lichtenberg, C. Schlensak, and F. Beyersdorf Stem cells used for cardiovascular tissue engineering. Eur. J. Cardiothorac. Surg., August 1, 2008; 34(2): 242 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yildirim, H. Naito, M. Didie, B. C. Karikkineth, D. Biermann, T. Eschenhagen, and W.-H. Zimmermann Development of a Biological Ventricular Assist Device: Preliminary Data From a Small Animal Model Circulation, September 11, 2007; 116(11_suppl): I-16 - I-23. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Morritt, S. K. Bortolotto, R. J. Dilley, X. Han, A. R. Kompa, D. McCombe, C. E. Wright, S. Itescu, J. A. Angus, and W. A. Morrison Cardiac Tissue Engineering in an In Vivo Vascularized Chamber Circulation, January 23, 2007; 115(3): 353 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sekine, T. Shimizu, J. Yang, E. Kobayashi, and T. Okano Pulsatile Myocardial Tubes Fabricated With Cell Sheet Engineering Circulation, July 4, 2006; 114(1_suppl): I-87 - I-93. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
