HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 73/4/840    most recent biolreprod.105.042788v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cho, Y. S. Articles by Hecht, N. B. Search for Related Content PubMed PubMed Citation Articles by Cho, Y. S. Articles by Hecht, N. B. Agricola Articles by Cho, Y. S. Articles by Hecht, N. B.

Meiotic Messenger RNA and Noncoding RNA Targets of the RNA-Binding Protein Translin (TSN) in Mouse Testis¹

Center for Research on Reproduction and Women's Health,³ University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 The Jackson Laboratory,⁴ Bar Harbor, Maine 04609

In postmeiotic male germ cells, TSN, formerly known as testis brain-RNA binding protein, is found in the cytoplasm and functions as a posttranscriptional regulator of a group of genes transcribed by the transcription factor CREM-tau. In contrast, in pachytene spermatocytes, TSN is found predominantly in nuclei. Tsn-null males show a reduced sperm count and high levels of apoptosis in meiotic cells, suggesting a critical function for TSN during meiosis. To identify meiotic target RNAs that associate in vivo with TSN, we reversibly cross-linked TSN to RNA in testis extracts from 17-day-old and adult mice and immunoprecipitated the complexes with an affinity-purified TSN antibody. Extracts from Tsn-null mice were used as controls. Cloning and sequencing the immunoprecipitated RNAs, we identified four new TSN target mRNAs, encoding diazepam-binding inhibitor-like 5, arylsulfatase A, a tetratricopeptide repeat structure-containing protein, and ring finger protein 139. In contrast to the population of postmeiotic translationally delayed mRNAs that bind TSN, these four mRNAs are initially expressed in pachytene spermatocytes. In addition, anti-TSN also precipitated a nonprotein-coding RNA (ncRNA), which is abundant in nuclei of pachytene spermatocytes and has a putative polyadenylation signal, but no open reading frame. A second similar ncRNA is adjacent to a GGA repeat, a motif frequently associated with recombination hot spots. RNA gel-shift assays confirm that the four new target mRNAs and the ncRNA specifically bind to TSN in testis extracts. These studies have, for the first time, identified both mRNAs and a ncRNA as TSN targets expressed during meiosis.

gametogenesis, gene regulation, meiosis, spermatogenesis, testis

² Correspondence: Norman B. Hecht, Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, 1310 Biomedical Research Building II/III, 421 Curie Boulevard, Philadelphia, PA 19104-6080. FAX: 215 573 5408; nhecht{at}mail.med.upenn.edu

This article has been cited by other articles:

				Z. Yu and N. B. Hecht The DNA/RNA-Binding Protein, Translin, Binds microRNA122a and Increases Its In Vivo Stability J Androl, September 1, 2008; 29(5): 572 - 579. [Abstract] [Full Text] [PDF]

				M. Xu, Y. You, P. Hunsicker, T. Hori, C. Small, M. D Griswold, and N. B Hecht Mice Deficient for a Small Cluster of Piwi-Interacting RNAs Implicate Piwi-Interacting RNAs in Transposon Control Biol Reprod, July 1, 2008; 79(1): 51 - 57. [Abstract] [Full Text] [PDF]

				J. S. Mattick A new paradigm for developmental biology J. Exp. Biol., May 1, 2007; 210(9): 1526 - 1547. [Abstract] [Full Text] [PDF]

				C. Kress, C. Gautier-Courteille, H. B. Osborne, C. Babinet, and L. Paillard Inactivation of CUG-BP1/CELF1 Causes Growth, Viability, and Spermatogenesis Defects in Mice Mol. Cell. Biol., February 1, 2007; 27(3): 1146 - 1157. [Abstract] [Full Text] [PDF]

				N. Iguchi, J. W. Tobias, and N. B. Hecht Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated PNAS, May 16, 2006; 103(20): 7712 - 7717. [Abstract] [Full Text] [PDF]