B. V. MCCLEARY (1)
(1) Megazyme International Ireland, Bray, County Wicklow, Ireland
2012 AACCI Annual Meeting
http://dx.doi.org/10.1094/CPLEX-2012-1112-05W
Traditionally, the term “soluble dietary fibre” would have related simply to soluble polysaccharides other than starch, i.e., polysaccharides that are not digested and absorbed in the human small intestine. However, it has been known for some time that non-digestible oligosaccharides (NDO) also act physiologically as dietary fibre. So now, the soluble dietary fibre also includes these carbohydrates. The recognition of NDO as dietary fibre (DF) means that these also must be measured in determining total dietary fibre. Several methods have been developed for the measurement of specific dietary fibres such as beta-glucan, fructan, and resistant starch, and other less specific procedures for Polydextrose and Fibersol 2. However, for regulatory purposes, measurement of total dietary fibre (including insoluble dietary fibre, high molecular weight soluble dietary fibre and low molecular weight soluble dietary fibre [i.e., NDO]), is generally all that is required. In this presentation, methods for the measurement of specific dietary fibres and of total dietary fibre will be described and discussed. Particular emphasis will be placed on problems in the measurement of NDO such as resistant maltodextrins and fructo-oligosaccharides.
Audio only mp3 >>
2012 AACCI Measurement of Soluble Dietary Fibre
