April 1997, Volume 10, Number 3 Pages 407-415
DOI: 10.1094/MPMI.1997.10.3.407
Research Note Identification of a Pathogenicity Locus, rpfA, in Erwinia carotovora subsp. carotovora That Encodes a Two-Component Sensor-Regulator Protein Reid D. Frederick,1,2 Jiliang Chiu,2 Jeffrey L. Bennetzen,1 and Autar K. Handa2 1Department of Biological Sciences and 2Department of Horticulture, Purdue University, West Lafayette, IN 47907 U.S.A.  Open Access.
A mutant of Erwinia carotovora subsp. carotovora, AH2552, created by a Mud1 insertion was found to be reduced in plant pathogenicity and deficient in extracellular protease and cellulase activity, although it produced normal levels of pectate lyase and polygalacturonase. A cosmid clone, pEC462, was isolated from a wild-type E. carotovora subsp. carotovora DNA library that concomitantly restored pathogenicity and protease and cellulase activities of AH2552 to wild-type levels when present in trans. The genetic locus that was disrupted in AH2552 by insertion of Mud1 has been designated rpfA, for regulator of pathogenicity factors. Sequencing of the rpfA region identified an open reading frame of 2,787 bp, and the predicted 929-amino acid polypeptide shared high identity with several two-component sensor-regulator proteins: BarA from Escherichia coli, ApdA from Pseudomonas fluorescens, PheN from P. tolaasii, RepA from P. viridiflava, LemA from P. syringae pv. syringae, and RpfC from Xanthomonas campestris pv. campestris. The RpfA locus described in this study encodes a putative sensor kinase protein that is involved in both extracellular protease and cellulase production and the pathogenicity of E. carotovora subsp. carotovora on potato tubers. Additional keywords: histidine protein kinase, pectolytic enzymes. Cited byPAS domain of the deduced Org35 protein mediates the interaction with NifAChinese Science Bulletin Jun 2006, Volume 51, Number 9: 1141-1144 CrossRef The PmrA-PmrB two-component system responding to acidic pH and iron controls virulence in the plant pathogen Erwinia carotovora ssp. carotovoraMolecular Microbiology Dec 2003, Volume 50, Number 3: 795-807 CrossRef Regulation of Erwinia carotovora hrpLEcc (sigma-LEcc), Which Encodes an Extracytoplasmic Function Subfamily of Sigma Factor Required for Expression of the HRP RegulonMolecular Plant-Microbe Interactions Sep 2002, Volume 15, Number 9: 971-980 Abstract
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| PDF with Links (274 KB) The hexY genes of Erwinia carotovora ssp. carotovora and ssp. atroseptica encode novel proteins that regulate virulence and motility co-ordinatelyEnvironmental Microbiology Jan 2000, Volume 1, Number 6: 535-547 CrossRef The Response Regulator ExpM Is Essential for the Virulence of Erwinia carotovora subsp. carotovora and Acts Negatively on the Sigma Factor RpoS (σS)Molecular Plant-Microbe Interactions Jul 1999, Volume 12, Number 7: 575-584 Abstract
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| PDF with Links (254 KB) Characterization of a novel RNA regulator of Erwinia carotovora ssp. carotovora that controls production of extracellular enzymes and secondary metabolitesMolecular Microbiology Aug 1998, Volume 29, Number 1: 219-234 CrossRef The rpfA gene of Xanthomonas campestris pathovar campestris, which is involved in the regulation of pathogenicity factor production, encodes an aconitaseMolecular Microbiology Jul 1998, Volume 28, Number 5: 961-970 CrossRef A newly identified regulator is required for virulence and toxin production in Pseudomonas syringaeMolecular Microbiology Jul 1998, Volume 28, Number 5: 917-929 CrossRef |
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