Evaluation of Damage in DNA Molecules Caused by Very-Low-Frequency Magnetic Fields Using Bacterial Cells

Akira HAGA
Yoshiaki KUMAGAI
Hidetoshi MATSUKI
Ginro ENDO
Akira IGARASHI
Koichiro KOBAYASHI

Publication
IEICE TRANSACTIONS on Communications   Vol.E88-B    No.8    pp.3249-3256
Publication Date: 2005/08/01
Online ISSN: 
DOI: 10.1093/ietcom/e88-b.8.3249
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Section of 2004 International Symposium on Electromagnetic Compatibility)
Category: Biological Effects
Keyword: 
mutation repairing gene,  umu operon,  very-low-frequency magnetic field (VLFMF),  Salmonella typhimurium TA1535 (pSK1002),  lacZ,  Y,  β-galactosidase,  

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Summary: 
The effect of intermediate frequency magnetic fields or, very-low-frequency magnetic fields (VLFMF) on living biological cells was investigated using a highly sensitive mutagenesis assay method. A bacterial gene expression system for mutation repair (umu system) was used for the sensitive evaluation of damage in DNA molecules. Salmonella typhimurium TA1535 (pSK1002) were exposed to VLFMF (20 kHz and 600 µT) in a specially designed magnetic field loading chamber. The experiment results showed the possibility of applying the umu assay for sensitive and effective evaluation of damage in DNA molecules. No effects from exposure to 20 kHz and 600 µT magnetic fields in terms of damage in DNA molecules were observed.