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Detection of Listeria monocytogenes in frozen food using a specific inlB virulence gene

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, , Citation Aula M. Al-Ghanim and Basil A. Abbas 2021 J. Phys.: Conf. Ser. 1879 022011 DOI 10.1088/1742-6596/1879/2/022011

1742-6596/1879/2/022011

Abstract

This study was undertaken to detect the presence of Listeria monocytogenes in frozen food. A total of 200 different food samples were collected from Basrah markets, southern Iraq, during September 2015 to March 2016. These samples included frozen fish, frozen burger, frozen chicken and worker's hands swabs, 50 each. The polymerase chain reaction technique was used to evaluate the presence of Listeria monocytogenes by using of inlB specific gene. The PCR results revealed that only four samples (7.27%) were contaminated by Listeria monocytogenes. In conclusion frozen food may carry a dangerous type of bacteria and lead to human illness.

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10.1088/1742-6596/1879/2/022011