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Unique Collaboration Charts the Migrations of a Parasite that Affected History
Researchers Sequence Louse DNA from Mummies and Propose New Model for its Development


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1 February 2005

Volume 191, Number 3
The Journal of Infectious Diseases 2005;191:453–460
0022-1899/2005/19103-0017$15.00
DOI: 10.1086/425936
MAJOR ARTICLE

Intracellular Macrophage Growth Rates and Cytokine Profiles of Mycobacterium tuberculosis Strains with Different Transmission Dynamics

Sue A. Theus,1

M. Donald Cave,2 and

Kathleen D. Eisenach1,3

1Departments of Pathology, 2Neurobiology and Developmental Science, and 3Microbiology-Immunology, Central Arkansas Veterans Healthcare System, University of Arkansas for Medical Sciences, Little Rock

Mycobacterium tuberculosis strains associated with IS6110 restriction fragment–length polymorphism (RFLP) pattern clusters and strains demonstrating unique IS6110 RFLP patterns were investigated in interferon-γ–activated THP-1 cells by measurement of binding, intracellular growth rate, and cytokine production. Binding was the same for all strains; however, strains from clusters grew significantly more rapidly than did unique strains. Maximal concentration of tumor necrosis factor (TNF)–α was detected at 2 days after infection, with unique strains eliciting significantly greater amounts than did strains from clusters. Interleukin (IL)–10 levels peaked at 1 day after infection with strains from clusters, whereas they peaked at 5 days after infection with unique strains. Rapid growth demonstrated by strains from clusters was highly correlated with rapid production of IL-10 and suppression of TNF-α in THP-1 cells during the early stages of infection. Characterization of this phenotype will further advance the investigation of virulence factors in M. tuberculosis.

Received 13 April 2004; accepted 24 June 2004; electronically published 30 December 2004.

Reprints or correspondence: Dr. Kathleen D. Eisenach, Central Arkansas Veterans Healthcare System, Medical Research Services, 151/LR, 4300 W. 7th St., Rm. GB-126, Little Rock, AR 72205 ().

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Online publication date: 1-Jan-2008.
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David W. Dunne, Anne Cooke. (2005) Opinion: A worm's eye view of the immune system: consequences for evolution of human autoimmune disease. Nature Reviews Immunology 5:5, 420-426
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  • Financial support: National Institutes of Health (grant N01 AI95383).

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