PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

doi:10.1084/jem.99.2.167

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The Journal of Experimental Medicine, Vol 99, 167-182, Copyright, 1954, by The Rockefeller Institute for Medical Research New York

ARTICLE

PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

R. Dulbecco M.D.¹ and Marguerite Vogt M.D.¹

¹ From the California Institute of Technology, Pasadena

Plaques have been produced with the three types of poliomyelitisviruses on monolayer tissue cultures of monkey kidney and monkeytestis. The number of plaques was proportional to the concentrationof the virus. Each plaque originates, therefore, from a singlevirus particle, defined as the virus unit that is unseparableby dilution. The plaques are due to the specific action of thevirus since they are suppressed by type-specific antiserum.

Purevirus lines were established by isolating the virus populationproduced in single plaques. These derived virus lines had thesame morphological, serological, and pathogenic properties asthe parent strain.

High titer virus stocks, with titers up to7 x 10⁸ plaque-forming particles per ml., were obtained.

Submitted on June 1, 1953

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