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Partial purification and characterization of Gigantocotyle explanatum somatic antigens

Published online by Cambridge University Press:  12 April 2024

G. Ahmad ,
M.K. Saifullah  and
W.A. Nizami
G. Ahmad*
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202 002, India
M.K. Saifullah
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202 002, India
W.A. Nizami
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202 002, India
*
*Address for correspondence: Malaria Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India Fax: +91 11 6162316 E-mail: gul_ahmad@yahoo.com
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Abstract

Soluble extracts of Gigantocotyle explanatum, isolated from the liver of buffalo Bubalus bubalis were fractionated on Sephadex G-200 columns. Nine major fractions referred to as F1, F2, F3, F4, F5, F6, F7, F8 and F9 were separated. Each fraction was tested by ELISA for antigenicity using sera from G. explanatum-infected field buffaloes. Fractions F1 and F2 were highly antigenic, F3, F4, F6 and F7 were moderately antigenic and F5, F8 and F9 were poorly antigenic. Analyses by SDS–PAGE revealed that each fraction comprised several polypeptide(s) in the molecular weight range of <29 to >205 kDa. Results of Western blotting indicated that not all polypeptides which appeared in the SDS–PAGE were antigenic. The antigenic molecules of each fraction were mostly in the low molecular weight range of <14 to >94 kDa with the polypeptides in the range of >14, 14, 18, 21–25 and 34–36 kDa.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 2 , June 2004 , pp. 95 - 99
Copyright
Copyright © Cambridge University Press 2004

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