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Effect of protein restriction on the messenger RNA contents of bone-matrix proteins, insulin-like growth factors and insulin-like growth factor binding proteins in femur of ovariectomized rats

Published online by Cambridge University Press:  09 March 2007

Yusuke Higashi ,
Asako Takenaka ,
Shin-Ichiro Takahashi  and
Tadashi Noguchi
Yusuke Higashi
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Asako Takenaka
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Shin-Ichiro Takahashi
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Tadashi Noguchi
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Division of Agriculture and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
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Abstract

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It has been reported that loss of ovarian oestrogen after menopause or by ovariectomy causes osteoporosis. In order to elucidate the effect of dietary protein restriction on bone metabolism after ovariectomy, we fed ovariectomized young female rats on a casein-based diet (50g/kg diet (protein restriction) or 200g/kg diet (control)) for 3 weeks and measured mRNA contents of bone-matrix proteins such as osteocalcin, osteopontin and α1 type I collagen, insulin-like growth factors (IGF) and IGF-binding proteins (IGFBP) in femur. Ovariectomy decreased the weight of fat-free dry bone and increased urinary excretion of pyridinium cross-links significantly, although dietary protein restriction did not affect them. Neither ovariectomy nor protein restriction affected the content of mRNA of osteopontin and osteocalcin; however, ovariectomy increased and protein restriction extensively decreased the α1 type I collagen mRNA content in bone tissues. Ovariectomy increased IGF-I mRNA only in the rats fed on the control diet. Conversely, protein rest riction increased and ovariectomy decreased the IGF-II mRNA content in femur. Furthermore, the contents of IGFBP-2, IGFBP-4 and IGFBP-5 mRNA increased, but the content of IGFBP-3 mRNA decreased in femur of the rats fed on the protein-restricted diet. In particular, ovariectomy decreased the IGFBP-2 mRNA content in the protein-restricted rats and the IGFBP-6 mRNA content in the rats fed on the control diet. These results clearly show that the mRNA for some of the proteins which have been shown to be involved in bone formation are regulated by both quantity of dietary proteins and ovarian hormones.

Keywords

BoneInsulin-like growth factorOvariectomyOsteoporosis
Type
Effect of protein restriction on rat bone
Information
British Journal of Nutrition , Volume 75 , Issue 6 , June 1996 , pp. 811 - 823
Copyright
Copyright © The Nutrition Society 1996

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