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Growth, body composition, hormonal and metabolic status in lambs treated long-term with growth hormone

Published online by Cambridge University Press:  09 March 2007

J. M. Pell ,
C. Elcock ,
R. L. Harding ,
D. J. Morrell ,
A. D. Simmonds  and
M. Wallis
J. M. Pell
Affiliation:
Endocrinology and Animal Physiology Department, AFRC Institute for Grassland and Animal ProductionHurley, Maidenhead, Berks SL6 5LR
C. Elcock
Affiliation:
Endocrinology and Animal Physiology Department, AFRC Institute for Grassland and Animal ProductionHurley, Maidenhead, Berks SL6 5LR
R. L. Harding
Affiliation:
School of Biological Sciences, University of Sussex, Falmer, Brighton, Sussex BNI 9QG
D. J. Morrell
Affiliation:
Institute of Child Health, 30 Guilford Street, London WCIN 1EH
A. D. Simmonds
Affiliation:
Endocrinology and Animal Physiology Department, AFRC Institute for Grassland and Animal ProductionHurley, Maidenhead, Berks SL6 5LR
M. Wallis
Affiliation:
School of Biological Sciences, University of Sussex, Falmer, Brighton, Sussex BNI 9QG
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Abstract

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The effect of long-term (10 weeks) treatment with growth hormone (GH) was investigated in twin lambs, one sibling being a control and the other treated with GH (0.1 mg/kg live weight per d). The lambs were fed on a concentrate-grass cube (9: 1 w/w) diet at a daily rate of 40 g fresh weightlkg Live weight. The average daily live-weight gain of the GH-treated lambs was 36% greater than that of the controls (307 v. 225 g/d, P < 0.01). The carcass composition of the GH-treated lambs changed: fat content was decreased (P < 001) and protein content was increased (P< 0.05) when expressed relative to carcass dry matter. The absolute weights and the weights when expressed relative to fleece-free empty body of some muscles were significantly increased in GH-treated lambs. The mean retention times of both particulate- and liquid-phase components of the digesta were unchanged by GH treatment, when calculated for the rumen or for the entire gastrointestinal tract. The feed conversion ratio was significantly greater (P < 0.01) in GH-treated lambs compared with controls. Nitrogen retained per g N intake was also significantly increased (P < 0.05) by GH treatment. Plasma urea concentrations were decreased (P < 005) and glucose concentrations were increased (P< 001) in GH-treated lambs, whereas non-esterified fatty acid concentrations were unchanged. Plasma insulin and total insulin-like growth factor-1 concentrations progressively increased in GH-treated lambs as treatment time continued. They were significantly correlated after week 4 of treatment. Two types of hepatic GH- binding site were detected, with high and low affinities for GH. The capacities of both binding sites were significantly increased (P < 0.05) in GH-treated lambs when expressed per unit microsomal protein but, when expressed per liver, only the capacity of the high-affinity site was increased.

Type
Growth, Body Composition
Information
British Journal of Nutrition , Volume 63 , Issue 3 , May 1990 , pp. 431 - 445
Copyright
Copyright © The Nutrition Society 1990

References

REFERENCES

Bauman, D. E., Eisemann, J. H. & Currie, W. B. (1982). Hormonal effects on partitioning of nutrients for tissue growth: role of growth hormone and prolactin. Federation Proceedings 41, 2538–2544.Google Scholar
Bittner, D. C. & McCleary, M. C. (1963). The cupric-phenanthurocene chelate in the determination of monosaccharides in whole blood. American Journal of Clinical Pathology 40, 423–431.Google Scholar
Braude, R. & Newport, M. J. (1973). Artificial rearing of pigs. British Journal of Nutrition 29, 447–455.CrossRefGoogle ScholarPubMed
Breier, B. H., Gluckman, P. D. & Bass, J. J. (1988). The somatotrophic axis in young steers: influence of nutritional status and oestradiol-17 β on hepatic high- and low-affinity somatotrophic binding sites. Journal of Endocrinology 116, 169–177.CrossRefGoogle ScholarPubMed
Brinklow, B. R. & Forbes, J. M. (1984). Effect of extended photoperiod on the growth of sheep. In Manipulation of Growth in Farm Animals, pp. 260–273 [Roche, J. F. and O'Callaghan, D., editors]. Boston: Martinus Nijhoff.Google Scholar
Brown, A. J., Coaltes, H. E. & Speight, B. S. (1978). A Photographic Guide to the Muscular and Skeletal Anatomy of the Beef Carcass. Bristol: AFRC Meat Research Institute.Google Scholar
Campbell, R. G., Steele, N. C., Caperna, T. J., McMurtry, J. P., Soloman, M. B. & Mitchell, A. D. (1988). Interrelationships between energy intake and endogenous porcine growth hormone administration on the performance, body composition and protein and energy metabolism of growing pigs weighing 25 to 55 kilograms live weight. Journal of Animal Science 66, 1643–1655.CrossRefGoogle ScholarPubMed
Chung, C. S. & Etherton, T. P. (1986). Characterization of porcine growth hormone (pGH) binding to porcine liver microsomes: chronic administration of pGH induces pGH binding. Endocrinorogy 119, 780–786.CrossRefGoogle ScholarPubMed
Daughaday, W. H., Kapadia, M. & Mariz, I. (1987). Serum somatomedin binding proteins: physiologic significance and interference in radioligand assay. Journal of Laboratory and Clinical Medicine 109, 355–363.Google ScholarPubMed
Daughaday, W. H., Parker, F. A., Borowsky, S., Trivedi, B. & Kapadia, M. (1982). Measurement of somatomedin-related peptides in fetal neonatal and maternal rat serum by insulin-like growth factor (IGF) I radioimmunoassay, IGF-II radioreceptor assay (RRA) and multiplication stimulating activity RRA after acid-ethanol extraction. Endocrinology 110, 575–581.CrossRefGoogle ScholarPubMed
D'Ercole, A. J. & Wilkins, J. R. (1984). Affinity labelled somatomedin-C binding proteins in rat serum. Endocrinology 114, 1141–1144.CrossRefGoogle Scholar
Dhanoa, M. S., Siddons, R. C., France, J. & Gale, D. C. (1985). A multicompartmental model to describe marker excretion patterns in ruminant faeces. British Journal of Nutrition 53, 663–671.CrossRefGoogle ScholarPubMed
Eisemann, J. H., Hammond, A. C., Bauman, D. E., Reynolds, P. J., McCutcheon, S. N., Tyrrell, H. F. & Haaland, G. L. (1986 b). Effect of bovine growth hormone administration on metabolism of growing Hereford heifers: protein and lipid metabolism and plasma concentrations of metabolites and hormones. Journal of Nutrition 116, 2504–2515.CrossRefGoogle ScholarPubMed
Eisemann, J. H., Hammond, A. C., Rumsey, T. S. & Bauman, D. E. (1989). Nitrogen and protein metabolism and metabolites in plasma and urine of beef steers treated with somatotropin. Journal of Animal Science 67, 105–115.CrossRefGoogle ScholarPubMed
Eisemann, J. H., Tyrrell, H. F., Hammond, A. C., Reynolds, P. J., Bauman, D. E., Haaland, G. L., McMurtry, J. P. & Varga, G. A. (1986 a). Effect of bovine growth hormone administration on metabolism of growing Hereford heifers: dietary digestibility, energy and nitrogen balance. Journal of Nutrition 116, 157–163.CrossRefGoogle ScholarPubMed
Fadlalla, A. M., Spencer, G. S. G. & Lister, D. (1985). The effect of passive immunization against somatostatin on marker retention time in lambs. Journal of Animal Science 61, 234–239.CrossRefGoogle ScholarPubMed
Florence, E. & Mitchell, K. G. (1972). A procedure for preparation of pig carcasses for chemical analysis with special reference to microanalysis. Proceedings of the British Society for Animal Production, pp. 101–107.Google Scholar
Gluckman, P. D., Butler, J. H. & Elliott, T. B. (1983). The ontogeny of somatotrophic binding sites in ovine hepatic membranes. Endocrinology 112, 1607–1612.CrossRefGoogle Scholar
Harding, R. L. (1989). Characterization and purification of receptors for growth hormone in normal and growth hormone-treated ruminants. PhD Thesis. University of Surrey.Google Scholar
Hart, I. C., Chadwick, P. M. E., Boone, T. C., angley, K. E., Rudman, C. & Souza, L. M. (1984). A comparison of the growth-promoting, lipolytic, diabetogenic and immunological properties of pituitary and recombinant DNA-derived bovine growth hormone (somatotropin). Biochemical Journal 224, 93–100.CrossRefGoogle ScholarPubMed
Hart, I. C., Flux, D. S., Andrews, P. & McNeilly, A. S. (1975). Radioimmunoassay for ovine and caprine growth hormone: its application to the measurement of basal circulating levels of growth hormone. Hormone and Metabolic Research 7, 35–40.CrossRefGoogle Scholar
Hart, I. C. & Johnsson, I. D. (1986). Growth hormone and growth in meat producing animals. In Control and Manipulation of Animal Growth, pp. 135–161 [Buttery, P. J., Haynes, N. B. and Lindsay, D. B., editors]. London: Butterworths.CrossRefGoogle Scholar
Hayden, T. J. & Smith, S. V. (1981). Effects of bromocriptine and occlusion of nipples on prolactin receptors and lactose synthetase activity in the mammary gland of the lactating rat. Journal of Endocrinology 91, 225–232.CrossRefGoogle ScholarPubMed
Johnsson, I. D., Hart, I. C. & Butler-Hogg, B. W. (1985). Effects of exogenous bovine growth hormone and bromocriptine on growth, body development, fleece weight and plasma concentrations of growth hormone, insulin and prolactin in female lambs. Animal Production 41, 207–217.Google Scholar
Johnsson, I. D., Hathorn, D. J., Wilde, R. M., Treacher, T. T. & Butler-Hogg, B. W. (1987). The effects of dose and method of administration of biosynthetic bovine somatotropin on live-weight gain, carcass composition and wool growth in young lambs. Animal Production 44, 405–414.Google Scholar
Lindahl, A., Isgaard, J., Carlsson, L. & Isaksson, O. G. P. (1987). Differential effects of insulin-like growth factor- I on colony formation and epiphyseal chondrocytes in suspension culture in rats of different ages. Endocrinology 121, 1061–1069.CrossRefGoogle ScholarPubMed
Martin, A. K. (1966). Some errors in the determination of nitrogen retention of sheep by nitrogen balance studies. Journal of Nutrition 20, 325–337.CrossRefGoogle ScholarPubMed
Millward, D. J. (1989). The endocrine response to dietary protein: the anabolic drive on growth. In Milk Proteins, pp, 49–61 [Barthe, C. A. and Schlimme, E., editors]. Darmstadt: Steinkopff Verlag.CrossRefGoogle Scholar
Muir, L. A., Wein, S., Duquette, P. F., Rickes, E. L. & Cordes, E. H. (1983). Effects of exogenous growth hormone and diethylstilbestrol on growth and carcass composition of growing lambs. Journal of Animal Science 56, 1315–1323.CrossRefGoogle ScholarPubMed
Murphy, L. J., Bell, G. I. & Friesen, H. G. (1987). Tissue distribution of insulin-like growth factors I and II mRNA in the adult rat. Endocrinology 120, 1279–1282.CrossRefGoogle ScholarPubMed
Pell, J. M. & Bates, P. C. (1987). Collagen and non-collagen protein turnover in skeletal muscle of growth hormone-treated lambs. Journal of Endocrinology 115, R1–R4.CrossRefGoogle ScholarPubMed
Pell, J. M., Blake, L. A., Elcock, C., Hathorn, D. J., Jones, A. R., Morrell, D. J. & Simmonds, A. D. (1987). Effect of growth hormone on IGF-1 concentrations, body composition and growth in lambs. Journal of Endocrinnlogy 112, Suppl., 63.Google Scholar
Peters, J. P. (1986). Consequences of accelerated gain and growth hormone administration for lipid metabolism in growing beef steers. Journal of Nutrition 116, 2490–2503.CrossRefGoogle ScholarPubMed
Ratcliffe, W. A., Chellard, G. S. & Ratcliffe, J. G. (1974). A critical evaluation of separation methods in radioimmunoassay for total triiodothyronine and thyroxine in unextracted human serum. Annals of Clinical Biochemistry 11, 224–229.CrossRefGoogle ScholarPubMed
Salacinski, P. R. P., McLean, C., Sykes, J. E. C., Clement-Jones, V. V. & Lowry, P. J. (1981). Iodination of proteins, glycoproteins and peptides using a solid phase oxidizing agent 1,3,4,6-tetrachloro-3,6-diphenyl glycouril (iodogen). Analytical Biochemistry 117, 136–146.CrossRefGoogle Scholar
Salmon, W. D. & Daughaday, W. H. (1957). A hormonally controlled serum factor which stimulates sulphate incorporation by cartilage in vitro. Journal of Laboratory and Clinical Medicine 49, 825–829.Google ScholarPubMed
Sandles, L. D. & Peel, C. J. (1987). Growth and carcass composition of prepubertal dairy heifers treated with bovine growth hormone. Animal Production 44, 21–27.Google Scholar
Scatchard, G. (1949). The attraction of proteins for small molecules and ions. Annals of the New York Academy of Science 51, 660–67.CrossRefGoogle Scholar
Spencer, G. S. G., Garsson, G. J. & Bergstrom, P. L. (1983). A novel approach to growth promotion using auto-immunisation against somatostatin. II. Effects on appetite, carcass composition and food utilisation in lambs. Livestock Production Science 10, 469–478.CrossRefGoogle Scholar
Struempler, A. W. & Burroughs, W. (1959). Stilbestrol feeding and growth hormone stimulation in immature ruminants. Journal of Animal Science 18, 427–436.CrossRefGoogle Scholar
Tindal, J. S., Blake, L. A., Simmonds, A. D. & Muzuno, H. (1982). Control of growth hormone release in goats: effect of vagal cooling, feeding and artificial distension of the rumen. Hormone and Metabolic Research 14, 425–429.CrossRefGoogle ScholarPubMed
Tindal, J. S., Knaggs, G. S., Hart, I. C. & Blake, L. A. (1978). Release of growth hormone in lactating and non-lactating goats in relation to behaviour, stages of sleep, electroencephalograms, environmental stimuli and levels of prolactin, insulin, glucose and free fatty acids in the circulation. Journal of Endocrinology 76, 333–346.CrossRefGoogle ScholarPubMed
Tsushima, T. & Friesen, H. G. (1973). Radioreceptor assay for growth hormone. Journal of Clinical Endocrinology and Metabolism 37, 334–337.CrossRefGoogle ScholarPubMed
Wagner, J. F. & Vennhuizen, E. L. (1978). Growth performance, carcass composition and plasma hormone levels in wether lambs when treated with growth hormone and thyrotropin. Journal of Animal Science 44, Suppl. 1, 397.Google Scholar
Wallace, A. L. C. & Bassett, J. M. (1966). Effect of sheep growth hormone on plasma insulin concentration in sheep. Metabolism 15, 95–97.CrossRefGoogle ScholarPubMed
Wallis, M. (1980). Receptors for growth hormone, prolactin, and the somatomedins. In Cellular Receptors for Hormones and Neurotransmitters, pp. 163–183 [Schulster, D. and Levitski, A., editors]. Chichester: John Wiley & Sons.Google Scholar
Wheatley, I. S., Wallace, A. L. C. & Bassett, J. M. (1966). Metabolic effects of ovine growth hormone in sheep. Journal of Endocrinology 35, 341–353.CrossRefGoogle ScholarPubMed
Zapf, J., Hauri, M., Waldvogel, M. & Froesch, E. R. (1986). Acute metabolic effects and half-lives of intravenously administered insulin-like growth factors I and II in normal and hypophysectomized rats. Journal of Clinical Investigation 77, 1768–1775.CrossRefGoogle Scholar