Skip to main content
SpringerLink
Log in

Efficient procedures for callus induction and adventitious shoot organogenesis in sugar beet (Beta vulgaris L.) breeding lines

  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Summary

Improved in vitro tissue culture systems are needed to facilitate the application of transgene technology to the improvement of sugar beet germplasms. Several commercially important sugar beet breeding lines (SDM, 3, 5, 8, 9, 10, 11, HB 526, and CMS 22003) and commercial varieties (Roberta and Gala) were tested for their regeneration capacity through adventitious shoot organogenesis from cotyledons, hypocotyls, root/hypocotyl/shoot transition zone tissues, and leaf lamina and petiole via an intervening callus phase. Callus induction and adventitious shoot regeneration was dependent on genotype and combinations of plant growth regulators. With cotyledon or hypocotyl explants, SDM 3 and 10 showed a better response on adventitious shoot regeneration in medium containing benzyladenine (BA) and 2,3,5-triiodobenzoic acid or 1-naphthaleneacetic acid (NAA) than SDM 11, 5, and 9. Shoot regeneration was obtained from hypocytyl-root or hypocotyl-shoot transition zone tissue in SDM 9, 10, and HB 526 grown on PGo medium supplemented with BA to induce callus, and the regeneration frequency was 25%. Adventitious shoots were also regenerated from leaf explants of SDM 3 and 9 cultured on medium containing NAA for callus induction and BA and NAA to induce shoot regeneration, and in SDM 10 and CSM 22003 cultured on medium containing BA for callus induction and to induce shoot regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Atanassov, A. I. Sugar beet. In: Evans, D. A.; Sharpe, W. R.; Ammirato, P. V., eds. Handbook of plant cell culture, vol. 4, New York: Macmillan Publishing Co.; 1986:652–689.

    Google Scholar 

  • Brandstatter, J.; Kieber, J. J. Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinins in Arabidopsis. Plant Cell 10:1009–1019; 1998.

    Article  PubMed  CAS  Google Scholar 

  • Catlin, D. W. The effect of antibiotics on the inhibition of callus induction and regeneration from cotyledons of sugarbeet. Plant Cell Rep. 9:285–288; 1990.

    Article  CAS  Google Scholar 

  • De Greef, W.; Jacobs, M. In vitro culture of the sugarbeet: description of a cell line with high regeneration capacity. Plant Sci. Lett., 17:55–61; 1979.

    Article  Google Scholar 

  • Detrez, C.; Tetu, T.; Sangwan, R. S.; Sangwan-Norreel, B. S. Direct organogenesis from petiole and thin cell layer explants in sugar beet cultured in vitro. J. Exp. Bot. 39:917–926; 1988.

    Article  CAS  Google Scholar 

  • D'Halluin, K.; Bossut, M.; Bonne, E.; Mazur, B.; Leemans, J.; Betterman, J. Transformation of sugarbeet (Beta vulgaris L.) and evaluation of herbicide resistance in transgenic plants. Bio/Technology 10:309–313; 1992.

    Article  Google Scholar 

  • Doley, W. P.; Saunders, J. W. Hormone-free medium will support callus production and subsequent shoot regeneration from whole plant leaf explants in some sugarbeet (Beta vulgaris L.) populations. Plant Cell Rep. 8:222–225; 1989.

    Article  Google Scholar 

  • Elliott, M. C.; Chen, D.-F.; Fowler, M. R.; Kirby, M. J.; Kubalakova, M.; Scott, N. W.; Slater, A. Transgenesis—a scheme for improving sugar beet productivity. Russian J. Plant Physiol. 43:544–551; 1996.

    CAS  Google Scholar 

  • Frank, M.; Rupp, H. M.; Prinsen, E.; Motyka, V.; Van Onckelen, H.; Schmulling, T. Hormone autotrophic growth and differentiation identifies mutant lines of Arabidopsis thaliana with altered cytokinin and auxin content or signaling. Plant Physiol. 122:721–730; 2000.

    Article  PubMed  CAS  Google Scholar 

  • Freytag, A. H.; Anand, S. C.; Rao-Arelli, A. P.; Owens, L. D. An improved medium for adventitious shoot formation and callus induction in Beta vulgaris L. in vitro. Plant Cell Rep. 7:30–34; 1988.

    Article  Google Scholar 

  • Grieve, T. M.; Gartland, K. M. A.; Elliott, M. C. Micropropagation of commercially important sugar beet cultivars. Plant Growth Regul. 21:15–18; 1997.

    Article  CAS  Google Scholar 

  • Hosemans, D.; Bossoutrot, D. Induction of haploid plants from in vitro culture of unpollinated beet ovules (Beta vulgaris L.). Z. Pflanzenzucht 91:74–77; 1983.

    Google Scholar 

  • Jacq, B.; Tetu, T.; Sangwan, R. S.; De Laat, A.; Sangwan-Norreel, B. S. Plant regeneration from sugarbeet (Beta vulgaris L.) hypocotyls cultured in vitro and flow cytometric nuclear DNA analysis of regenerants. Plant Cell Rep. 11:329–333; 1992.

    Article  Google Scholar 

  • Jacq, B.; Tetu, T.; Sangwan, R. S.; Sangwan-Norreel, B. S. Efficient production of uniform plants from cotyledon explants of sugarbeet (Beta vulgaris L.). Plant Breed. 110:185–191; 1993.

    Article  Google Scholar 

  • Kakimoto, T. CKI1, a histidine kinase homolog implicated in cytokinin signal transduction. Science 274:982–985; 1996.

    Article  PubMed  CAS  Google Scholar 

  • Kubalakova, M. Somatic embryogenesis and cytoplasmic sterility in Beta vulgaris L. var. Saccharifera. Biol. Plant. 32:414–419; 1990.

    Google Scholar 

  • Levall, M. W.; Bornman, J. F. Selection in vitro for UV-tolerant sugarbeet (Beta vulgaris) somaclones. Physiol. Plant. 88:37–43; 1993.

    Article  CAS  Google Scholar 

  • Liu, C.-M.; Xu, Z.-H.; Chua, N.-H. Auxin polar transport is essential for the establishment of bilateral symmetry during early plant embryogenesis. Plant Cell 5:621–630; 1963.

    Article  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Thidiazuron induced somatic embryogenesis in intact seedling of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons. Physiol. Plant. 94:268–276; 1995.

    Article  CAS  Google Scholar 

  • Pedersen, M. G.; Andersen, J. M. Callus morphology and endogenous cytokinin in regenerating sugar beet callus cultures. Proc. Beta Eucarpia Meeting, Helsingor; 1988.

  • Pua, E. C.; Thorpe, T. A. Differential response of non-selected and Na2SO4-selected callus cultures of Beta vulgaris L. to salt stress. J. Plant Physiol. 123:241–248; 1986.

    CAS  Google Scholar 

  • Ritchie, G. A.; Short, K. C.; Davey, M. R. In vitro shoot regeneration from callus, leaf axils and petioles of sugar beet (Beta vulgaris L.). J. Exp. Bot. 40:277–283; 1989.

    Article  Google Scholar 

  • Roussy, I.; Dubois, F.; Sangwan, R. S.; Sangwan-Norreel, B. S. In planta 2,3,5-triiodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet (Beta vulgaris L.) plants. Plant Cell Rep. 16:142–146; 1996.

    CAS  Google Scholar 

  • Rupp, H. M.; Frank, M.; Werner, T.; Strnad, M.; Schmulling, T. Increased steady mRNA levels of the STM and KNAT1 homeobox genes in cytokinin overproducing Arabidopsis thaliana indicate a role for cytokinins in the shoot apical meristem. Plant J. 18:557–563; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Saunders, J. W.; Acquaah, G.; Renner, K. A.; Doley, W. P. Monogenic dominant sulfonylurea resistance in sugarbeet from somatic cell selection. Crop Sci. 32:1357–1360; 1992.

    Article  CAS  Google Scholar 

  • Saunders, J. W.; Doley, W. P. One step shoot regeneration from callus of whole plant leaf explants of sugarbeet lines and a somaclonal variation for in vitro culture behaviour. J. Plant Physiol. 124:473–479; 1986.

    CAS  Google Scholar 

  • Steel, R. G. D.; Torrie, J. H. Principles and procedures of statistics, a biometrical approach, 2nd edn. New York: McGraw Hill; 1980.

    Google Scholar 

  • Tenning, P.; Weich, E. W.; Kjarsgaard, U.-B.; Lelu, M.-A.; Nihlgard, M. Somatic embryogenesis from zygotic embryos of sugarbeet (Beta vulgaris L.). Plant Sci. 81:103–109; 1992.

    Article  Google Scholar 

  • Tetu, T.; Sangwan, R. S.; Sangwan-Norreel, B. S. Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus. J. Exp. Bot. 38:506–517; 1987.

    Article  CAS  Google Scholar 

  • Van Geyt, J.; Speckmann, G. J. Jr.; D'Halluin, K.; Jacobs, M. In vitro induction of haploid plants from unpollinated ovules and ovaries of the sugarbeet (Beta vulgaris L.). Theor. Appl. Genet. 73:920–925; 1987.

    Article  Google Scholar 

  • Yu, M. H. Growth and reproduction performance of ovule-induced sugarbeet plants. SABRAO J. 24:47–55; 1992.

    Google Scholar 

  • Zhang, C.-L.; Chen, D.-F.; Elliott, M. C.; Slater, A. TDZ-induced organogenesis and somatic embryogenesis in sugar beet (Beta vulgaris L.). In Vitro Cell. Dev. Biol. Plant 37:305–310; 2001a.

    Article  CAS  Google Scholar 

  • Zhang, C.-L.; Chen, D.-F.; McCormac, A. C.; Scott, N. W.; Elliott, M. C.; Slater, A. Use of the GFP reporter as a vital marker for Agrobacterium-mediated transformation of sugar beet (Beta vulgaris L.). Mol. Biotech. 17:109–117; 2001b.

    Article  Google Scholar 

  • Zhong, Z.; Smith, H. G.; Thomas, T. H. In vitro culture of petioles and intact leaves of sugar beet (Beta vulgaris). Plant Growth Regul. 12:59–66; 1993.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Norman Borlaug Institute for Plant Science Research, Faculty of Health and Life Sciences, De Montfort University, The Gateway, LE1 9BH, Leicester, UK

    Chun-Lai Zhang, Dong-Fang Chen, Malcolm C. Elliott & Adrian Slater

Authors
  1. Chun-Lai Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dong-Fang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Malcolm C. Elliott
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Adrian Slater
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chun-Lai Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, CL., Chen, DF., Elliott, M.C. et al. Efficient procedures for callus induction and adventitious shoot organogenesis in sugar beet (Beta vulgaris L.) breeding lines. In Vitro Cell.Dev.Biol.-Plant 40, 475–481 (2004). https://doi.org/10.1079/IVP2004563

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1079/IVP2004563

Key words

Search

Navigation