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Protein turnover of breast muscle in germ-free and conventional chicks

Published online by Cambridge University Press:  09 March 2007

T. Muramatsu ,
D. N. Salter  and
M. E. Coates
T. Muramatsu
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
D. N. Salter
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
M. E. Coates
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
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Abstract

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1. The effect of the gut microflora on protein turnover in pectoral muscle (M. pectoralis profundus) was studied by means of dietary infusion of L-[UJ-14C]phenylalanine and of massive dose injection of L-[4-3H]phenylalanine in chicks fed on a semi-purified casein—gelatin (SCG) diet until 19 d of age, and in those subsequently given either a nitrogen-free (NF) diet or NF supplemented with methionine and arginine (MA) for a further 9 d.

2. Time-course changes in radioactivity released in expired carbon dioxide during the 8 h infusion period showed that isotopic equilibrium was reached in 4 h with the SCG diet and in 5 h with the MA diet. However, with the protein-deprived chicks given the NF diet, isotopic equilibrium was not achieved since radioactivity in CO2 increased linearly throughout.

3. On feeding the NF diet, fractional protein synthesis rate and the absolute amount of protein synthesized in chick breast muscle were reduced. These reductions were partially alleviated by supplementing the NF diet with methionine and arginine.

4. The fractional degradation rate of breast muscle was increased in chicks given the NF diet, while the absolute amount of protein degraded was decreased. The addition of methionine and arginine counteracted these changes brought about by protein starvation.

5. Generally speaking, the presence of the gut microflora had little, if any, effect on protein turnover rate in chick-breast muscle.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 54 , Issue 1 , July 1985 , pp. 131 - 145
Copyright
Copyright © The Nutrition Society 1985

References

Coates, M. E., Fuller, R., Harrison, G. F., Lev, M. & Suffolk, S. F. (1963). British Journal of Nutrition 17, 141–150.CrossRefGoogle Scholar
Featherston, W. R. & Horn, G. W. (1973). Journal of Nutrition 103, 757–765.CrossRefGoogle Scholar
Fowler, V. R. (1980). In Growth in animals, pp. 249–263. [Lawrence, T. L. J., editor]. London: Butterworths.CrossRefGoogle Scholar
Fuller, R. (1968). In The Germ-free Animal in Research, pp. 37–45 [Coates, M. E., editor]. London: Academic Press.Google Scholar
Garlick, P. J., Burk, T. L. & Swick, R. W. (1976). American Journal of Physiology 230, 1108–1112.CrossRefGoogle Scholar
Garlick, P. J., Fern, M. & Preedy, V. R. (1983). Biochemical Journal 210, 669–676.CrossRefGoogle Scholar
Garlick, P. J., McNurlan, M. A. & Preedy, V. R. (1980). Biochemical Journal 92, 719–723.CrossRefGoogle Scholar
Garlick, P. J., Millward, D. J. & James, W. P. T. (1973). Biochemical Journal 136, 935–945.CrossRefGoogle Scholar
Garlick, P. J., Millward, D. J., James, W. P. T. & Waterlow, J. C. (1975). Biochimica et Biophysica Acta 414, 71–84.CrossRefGoogle Scholar
Giles, K. W. & Myers, A. (1965). Nature 206, 93.CrossRefGoogle Scholar
Goldberg, A. L. & Chang, T. W. (1978). Federation Proceedings 37, 2301–2307.Google Scholar
Goldspink, D. F. (1977). Journal of Physiology 264, 283–296.CrossRefGoogle Scholar
Harney, M. E., Swick, R. W. & Benevenga, N. J. (1976). American Journal of Physiology 231, 1018–1023.CrossRefGoogle Scholar
Laurent, G. J., Sparrow, M. P. & Millward, D. J. (1978). Biochemical Journal 176, 407–417.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Journal of Biological Chemistry 193, 265–275.CrossRefGoogle Scholar
McCaman, M. W. & Robins, E. (1962). Journal of Laboratory and Clinical Medicine 59, 885–890.Google Scholar
MacDonald, M. L. & Swick, R. W. (1981). Biochemical Journal 194, 811–819.CrossRefGoogle Scholar
McFarlane, I. G. & von Holt, C. (1969). Biochemical Journal 111, 557–563.CrossRefGoogle Scholar
McNurlan, M. A., Tomkins, A. M. & Garlick, P. J. (1979). Biochemical Journal 178, 373–379.CrossRefGoogle Scholar
Maruyama, K., Sunde, M. L. & Swick, R. W. (1978). Biochemical Journal 176, 573–582.CrossRefGoogle Scholar
Millward, D. J., Garlic, P. J., Nnanyelugo, D. O. & Waterlow, J. C. (1976). Biochemical Journal 156, 185–188.CrossRefGoogle Scholar
Millward, D. J. & Waterlow, J. C. (1978). Federation Proceedings 37, 2283–2290.Google Scholar
Mitchell, H. H. (1959). In Protein and Amino Acid Nutrition, pp. 11–43 [Albanese, A. A., editor]. New york: Academic Press.Google Scholar
Munro, H. N. & Fleck, A. (1969). In Mammalian protein metabolism, vol. 3, pp. 443–525 [Munro, H. N., editor]. New York and London: Academic Press.Google Scholar
Muramatsu, T., Coates, M. E., Hewitt, D., Salter, D. N. & Garlick, P. J. (1983). British Journal of Nutrition 49, 453–462.CrossRefGoogle Scholar
Muramatsu, T. & Okumura, J. (1979 a). Nutrition Reports International 19, 335–342.Google Scholar
Muramatsu, T. & Okumura, J. (1979 b). Nutrition Reports International 20, 709–716.Google Scholar
Muramatsu, T. & Okumura, J. (1980). Journal of Nutrition 110, 59–65.CrossRefGoogle Scholar
Okumura, J., Hewitt, D. & Coates, M. E. (1978). British Journal of Nutrition 39, 99–104.CrossRefGoogle Scholar
Reeds, P. J. (1974). British Journal of Nutrition 31, 259–270.CrossRefGoogle Scholar
Rolls, B. A., Turvey, A. & Coates, M. E. (1978). British Journal of Nutrition 39, 91–98.CrossRefGoogle Scholar
Schimke, R. T. (1962). Journal of Biological Chemistry 237, 1921–1924.CrossRefGoogle Scholar
Scholz, R. W. & Featherston, W. R. (1969). Journal of Nutrition 98, 193–201.CrossRefGoogle Scholar
Yokogoshi, H. & Yoshida, A. (1980). Journal of Nutrition 110, 375–382.CrossRefGoogle Scholar
Young, V. R. & Alexis, S. D. (1968). Journal of Nutrition 96, 255–262.CrossRefGoogle Scholar
Young, V. R., Chen, S. C. & MacDonald, J. (1968). Biochemical Journal 106, 913–919.CrossRefGoogle Scholar