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Photosensitization of albino rats fed on lucerne-proetin concentrate

Published online by Cambridge University Press:  07 January 2011

Evelyn Lohrey ,
B. Tapper  and
E. L. Hove
Evelyn Lohrey
Affiliation:
Applied Biochemistry Division DSIR, Palmerston North, New Zealand
B. Tapper
Affiliation:
Applied Biochemistry Division DSIR, Palmerston North, New Zealand
E. L. Hove
Affiliation:
Applied Biochemistry Division DSIR, Palmerston North, New Zealand
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Abstract

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1. The photosensitizing effect of leaf-protein concentrate (LPC) prepared from lucerne (Medicago sativa) was demonstrated when it was included in the diet of albino rats at concentrations between 40 and 480 g/kg diet.

2. Skin lesions of varying severity up to the sloughing of ears and tails occurred when such rats were illuminated with natural daylight through window-glass or with simulated daylight from a bank of lamps having emission maxima at 410, 437 and 660 nm and with approximately 4% of the energy of summer sunlight.

3. Protein concentrates prepared from ryegrass (Lolium multiforum) and a crude chlorophyll extract of spinach (Spinacea oleracea) did not cause these effects.

4. Extracts from blood plasma and livers of rats given lucerne LPC contained pheophorbide-a and two other unidentified green pigments, but no detectable phylloerythrin or chlorophyll. Extracts from the blood of rats given ryegrass LPC had no detectable pheophorbide or other chlorophyll-derived pigments.

5. The lucerne LPC given to the rats contained relatively large amounts of pheophorbide-a among a variety of pigments. The ryegrass LPC and the spinach extracts had only small amounts of this pigment relative to the other chlorophyll-type pigments.

6. These findings indicate that pheophorbide-a and related pigments are the agents responsible for the photosensitization of the rats. Possible explanations of their presence in lucerne LPC are discussed.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 31 , Issue 2 , March 1974 , pp. 159 - 166
Copyright
Copyright © The Nutrition Society 1974

References

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