Molecular & Cellular Proteomics
ResearchThe Proteome of Pig Spermatozoa Is Remodeled During Ejaculation*[S]
Graphical Abstract
Highlights
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Proteome of mature boar spermatozoa from cauda epididymal and ejaculated sources were analyzed by iTRAQ-based LC-MS/MS.
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1,723 sperm proteins identified (974 of Sus scrofa taxonomy).
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1,602 sperm proteins quantified (960 of Sus scrofa taxonomy).
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32 Sus scrofa sperm proteins were differentially expressed among sperm sources.
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The proteome of boar spermatozoa is remodelled during ejaculation.
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Author contributions: C.P.-P., I.P., J.L., and I.B. performed research; C.P.-P. wrote the paper; I.P. and J.R. designed research; E.A.M., H.R.-M., and J.R. contributed new reagents/analytic tools; H.R.-M. and J.R. analyzed data; E.A.M., H.R.-M., and J.R. final draft review.
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This study was supported by MINECO (Spain) and FEDER funds (EU) (AGL2015-69738-R), and Seneca Foundation Murcia, Spain (19892/GERM-15); FORSS (745971) and The Swedish Research Council FORMAS (2017-00946), Stockholm, Sweden. C. Perez-Patiño, I. Barranco and J. Li were financially supported by Seneca Foundation (Murcia, Spain), MECD (Madrid, Spain) and the China Scholarship Council, respectively.
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This article contains supplemental Figures and Data. The authors declare no conflicts of interest regarding this study.
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- iTRAQ
isobaric tags for relative and absolute quantification
- SRF
sperm-rich ejaculate fraction
- AI
artificial insemination
- VTH
veterinary teaching hospital
- SP
seminal plasma
- TEAB
triethylammonium bicarbonate buffer
- TCEP
2-carboxyethyl phosphine
- MMTS
S-methyl methanethiosulphonate
- FA
formic acid
- FDR
false discovery rate
- PCA
principal component analysis
- MeV
multi-experiment viewer
- FC
fold change
- GO
gene ontology
- DDI1
DNA damage inducible 1 homolog 1
- CES1
Carboxyl ester hydrolase
- pB1
porcine B1
- BSP
bovine seminal plasma
- FN1
Fibronectin 1
- TSP1
Thrombospondin-1
- ROS
reactive oxygen species
- WGA
wheat germ agglutinin
- COL18A1
type XVIII Collagen α1 chain.
The abbreviations used are: