Molecular & Cellular Proteomics
Technological Innovation and ResourcesMS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting*
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Work in AS and NLV laboratories was supported by MPG core funding. AS laboratory was supported by KFO249 and TRR83 (Project A17) grants from Deutsche Forschungsgemeinschaft; NLV laboratory by Human Frontier Science Program Career Development Award (CDA00060/2012). Work in the FB laboratory was supported by the Excellence Initiative of the German Federal and State Governments (Institutional Strategy, measure ‘support the best ZUK 64‘). MK and SRJ are recipients of IMPRS PhD student fellowship.
This article contains supplemental material.
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Present address: Center for Molecular and Cellular Bioengineering of TU Dresden, 01307 Dresden, Germany.
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Present address: IMP Research Institute of Molecular Pathology, 1030 Vienna, Austria.
Author contributions: M.K., M.G., and A.S. designed the study. M.K., A.B., and D.D. designed and produced protein chimeras. M.K. performed MS Western benchmarking and validation experiments and quantified zebrafish histones. S.R.J. and N.L.V. performed experiments with zebrafish embryos. M.A. and F.B. performed KD experiments in HeLa cells. M.K. and A.S. wrote the draft that was jointly revised by all coauthors.