Journal of Biological Chemistry
Editors' PicksMesencephalic astrocyte–derived neurotrophic factor is an ER-resident chaperone that protects against reductive stress in the heart
Cited by (0)
This article contains supporting information.
Author contributions—A. A. and C. C. G. conceptualization; A. A., E. A. B., W. T. S., M. S. D., A. S. B., D. J. T., A. N. P., C. A., A. V. S., S. D., and C. C. G. data curation; A. A., E. A. B., W. T. S., A. S. B., D. J. T., A. N. P., A. V. S., S. D., and C. C. G. formal analysis; A. A., E. A. B., and C. C. G. funding acquisition; A. A., E. A. B., W. T. S., A. S. B., D. J. T., and C. C. G. validation; A. A., E. A. B., S. D., and C. C. G. investigation; A. A. and C. C. G. visualization; A. A., E. A. B., S. D., and C. C. G. methodology; A. A. and C. C. G. writing-original draft; A. A., E. A. B., W. T. S., D. J. T., S. D., and C. C. G. writing-review and editing; D. J. T., S. D., and C. C. G. supervision; C. C. G. project administration.
Funding and additional information—This work was supported by American Heart Association Grant 17PRE33670796; National Institutes of Health Grant 1F31HL140850 (to E. A. B.) and Grants R01 HL135893, R01 HL141463, and R01 HL149931 (to C. C. G.); the San Diego State University (SDSU) Heart Institute (to W. T. S., A. A., E. A. B., and C. C. G.); the Inamori Foundation (to E. A. B.); and the ARCS Foundation, Inc., San Diego Chapter (to W. T. S. and E. A. B). Additionally, W. T. S., A. A., and E. A. B are Rees-Stealy Research Foundation Phillips Gausewitz, M.D., Scholars of the SDSU Heart Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Abbreviations—The abbreviations used are:
- ER
endoplasmic reticulum
- AAV9
adeno-associated virus serotype 9
- ANOVA
analysis of variance
- ATF6
activating transcription factor 6α
- GRP78 and -94
78- and 94-kDa glucose-regulated protein, respectively
- HMGB1
high-mobility group box 1 protein
- I/R
ischemia/reperfusion
- LVDP
left ventricular developed pressure
- MANF
mesencephalic astrocyte derived neurotrophic factor
- MTT
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
- NEM
N-ethylmaleimide
- sI
simulated ischemia
- sI/R
simulated ischemia/reperfusion
- TG
thapsigargin
- TTC
2,3,5-triphenyl tetrazolium chloride
- UPR
unfolded protein response
- aa
amino acids
- KD
knockdown
- AMVM
adult mouse ventricular myocyte
- NRVM
neonatal rat ventricular myocyte
- PI
propidium iodide
- ROS
reactive oxygen species
- α1AT
α1-antitrypsin
- IP
immunoprecipitation
- IB
immunoblotting
- rMANF
recombinant MANF
- TM
tunicamycin
- DMEM
Dulbecco's modified Eagle's medium
- FBS
fetal bovine serum
- AdV
adenovirus
- qPCR
quantitative PCR
- DPBS
Dulbecco's PBS
- HA
hemagglutinin
- rLA
reduced α-lactalbumin
- GAPDH
glyceraldehyde-3-phosphate dehydrogenase
- LDH
lactate dehydrogenase
- MEM
minimal essential medium.