Journal of Biological Chemistry
Protein Structure and FoldingProinsulin Is Refractory to Protein Fibrillation: TOPOLOGICAL PROTECTION OF A PRECURSOR PROTEIN FROM CROSS-β ASSEMBLY*
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This work was supported by National Institutes of Health Grant DK0697674 (to M. A. W.). This article is a contribution from the Cleveland Center for Structural Biology. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at http://www.jbc.org) contains five figures providing high-performance liquid chromatography purification profile of split proinsulin analogs, CD spectra of insulin, proinsulin, and insulin analogs at fibril-promoting conditions (pH 2.0 and 65 °C), CD difference spectra, kinetic profile of B-chain fibrillation, and control studies of the kinetics of ThT fluorescence enhancement in relation to optical scattering and EM morphology.
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