Journal of Biological Chemistry
Volume 294, Issue 47, 22 November 2019, Pages 17725-17734
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Cell Biology
Glucose starvation induces mitochondrial fragmentation depending on the dynamin GTPase Dnm1/Drp1 in fission yeast

https://doi.org/10.1074/jbc.RA119.010185Get rights and content
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Mitochondria undergo morphological and dynamic changes in response to environmental stresses. Few studies have focused on addressing mitochondrial remodeling under stress. Using the fission yeast Schizosaccharomyces pombe as a model organism, here we investigated mitochondrial remodeling under glucose starvation. We employed live-cell microscopy to monitor mitochondrial morphology and dynamics of cells in profusion chambers under glucose starvation. Our results revealed that mitochondria fragment within minutes after glucose starvation and that the dynamin GTPase Dnm1 is required for promoting mitochondrial fragmentation. Moreover, we found that glucose starvation enhances Dnm1 localization to mitochondria and increases the frequency of mitochondrial fission but decreases PKA activity. We further demonstrate that low PKA activity enhances glucose starvation–induced mitochondrial fragmentation, whereas high PKA activity confers resistance to glucose starvation–induced mitochondrial fragmentation. Moreover, we observed that AMP-activated protein kinase is not involved in regulating mitochondrial fragmentation under glucose starvation. Of note, glucose starvation–induced mitochondrial fragmentation was associated with enhanced reactive oxygen species production. Our work provides detailed mechanistic insights into mitochondrial remodeling in response to glucose starvation.

mitochondria
stress
glucose
dynamin
fungi

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This work was supported by National Key Research and Development Program of China Grant 2018YFC1004700; National Natural Science Foundation of China Grants 91754106, 31871350, 31671406, 31601095, and 31621002; Strategic Priority Research Program of the Chinese Academy of Sciences Grant XDB19040101; Major/Innovative Program of Development Foundation of the Hefei Center for Physical Science and Technology Grant 2017FXCX008; and China's 1000 Young Talents Recruitment Program. The authors declare that they have no conflicts of interest with the contents of this article.

This article contains Figure S1 and Tables S1 and S2.

1

Both authors contributed equally to this work.

2

Present address: Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.