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J. Biol. Chem., Vol. 280, Issue 15, 14723-14732, April 15, 2005

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GRIF-1 and OIP106, Members of a Novel Gene Family of Coiled-Coil Domain Proteins

ASSOCIATION IN VIVO AND IN VITRO WITH KINESIN^*

Kieran Brickley, Miriam J. Smith, Mike Beck, and F. Anne Stephenson

From the Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, United Kingdom

-Aminobutyric acid_A receptor-interacting factor (GRIF-1) is a 913-amino acid protein proposed to function as a GABA_A receptor ₂ subunit-interacting, trafficking protein. GRIF-1 shares 44% amino acid sequence identity with O-linked N-acetylglucosamine transferase interacting protein 106, OIP106. Both proteins contain predicted coiled-coil domains and probably constitute a novel gene family. The Drosophila orthologue of this family of proteins may be Milton. Milton shares 44% amino acid homology with GRIF-1. Milton is proposed to function in kinesin-mediated transport of mitochondria to nerve terminals. We report here that GRIF-1 and OIP106 also associate with kinesin and mitochondria. Following expression in human embryonic kidney 293 cells, both GRIF-1 and OIP106 were shown by co-immunoprecipitation to be specifically associated with an endogenous kinesin heavy chain species of 115 kDa and exogenous KIF5C. Association of GRIF-1 with kinesin was also evident in native brain and heart tissue. In the brain, anti-GRIF-1-(8–633) antibodies specifically co-immunoprecipitated two kinesin-immunoreactive species with molecular masses of 118 and 115 kDa, and in the heart, one kinesin-immunoreactive species, 115 kDa, was immunoprecipitated. Further studies revealed that GRIF-1 was predominantly associated with KIF5A in the brain and with KIF5B in both the heart and in HEK 293 cells. Yeast two-hybrid interaction assays and immunoprecipitations showed that GRIF-1 associated directly with KIF5C with the GRIF-1/KIF5C interaction domain localized to GRIF-1-(124–283). These results further support a role for GRIF-1 and OIP106 in protein and/or organelle transport in excitable cells in a manner analogous to glutamate receptor-interacting-protein 1, in the motor-dependent transport of -amino-3-hydroxy-5-methylisoxazole-4-propionate glutamate excitatory neurotransmitter receptors to dendrites.

Received for publication, August 9, 2004 , and in revised form, December 21, 2004.

^* This work was funded by the Biotechnology and Biological Sciences Research Council, United Kingdom. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Max-Planck-Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden D-01307, Germany.

To whom correspondence should be addressed: Dept. of Pharmaceutical and Biological Chemistry, School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, United Kingdom. Tel.: 44-207-753-5877; Fax: 44-207-753-5964; E-mail: anne.stephenson{at}ulsop.ac.uk.

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