Journal of Biological Chemistry
Protein Synthesis and DegradationA Conserved C-terminal Element in the Yeast Doa10 and Human MARCH6 Ubiquitin Ligases Required for Selective Substrate Degradation*
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This work was supported by National Institutes of Health Grant R01 GM046904 (to M. H.). Additional training support was provided by National Institutes of Health Grants F32 GM113456 (to J. M. B.) and T32 GM7223 (to D. Z.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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S. G. Kreft and M. Hochstrasser, unpublished data.
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Both authors made equal contributions to this work.
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Present address: Program in Structural Biology, Sloan Kettering Inst., 1275 York Ave., New York, NY 10065.
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The abbreviations used are:
- ER
endoplasmic reticulum
- ERAD
ER-associated protein degradation
- CTE
C-terminal element
- CRISPR
clustered regularly interspaced short palindromic repeats
- SD
synthetic defined
- CHX
cycloheximide
- ssODN
single-stranded donor oligonucleotide
- TM
transmembrane helix
- TD
TEB4-Doa10
- MARCH
membrane-associated RING-CH
- INM
inner nuclear membrane
- gRNA
guide RNA.