HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) A correction has been published Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lustigman, S. Articles by Brotman, B. Search for Related Content PubMed PubMed Citation Articles by Lustigman, S. Articles by Brotman, B. Social Bookmarking                      What's this?

Volume 271, Number 47, Issue of November 22, 1996 pp. 30181-30189
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Cloning of a Cysteine Protease Required for the Molting of Onchocerca volvulus Third Stage Larvae

(Received for publication, April 16, 1996, and in revised form, August 30, 1996)

Sara Lustigman , James H. McKerrow ^¶ , Kashmira Shah , Jing Lui , Tellervo Huima , Matt Hough ^¶ and Betsy Brotman

From the  Laboratory of Virology and Parasitology, The Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York 10021, the ^¶ Department of Pathology, University of California and Department of Veterans Affairs Medical Center, San Francisco, California 94121, and  Vilab II, The Liberian Institute for Biomedical Research, Robertsfield, Liberia

We have investigated the involvement of a cysteine protease in the development of Onchocerca volvulus fourth stage larvae (L4) by testing the effect of cysteine protease inhibitors on the survival of third stage larvae (L3), and the molting of L3 to L4 in vitro. When larvae were cultured in the presence of specific inhibitors, the peptidyl monofluoromethylketones, viability of either L3 or L4 was not affected. However, the inhibitors reduced the number of L3 that molted to L4 in vitro in a time- and dose-dependent manner. Molting was completely inhibited in the presence of 50-250 µM inhibitor. Ultrastructural examination of L3 that did not molt in the presence of inhibitors indicated that new L4 cuticle was synthesized, but there was no separation between the L3 and the L4 cuticles. The endogenous cysteine protease was detected in molting larvae after binding to labeled inhibitors, and by antibodies directed against a recombinant O. volvulus L3 cysteine protease that was cloned and expressed. The enzyme was detected in cuticle regions where the separation between the cuticles occurs in molting larvae. These studies suggest that molting and successful development of L4 depends on the expression and release of a cysteine protease.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Kumar, K. Kumar, R. Korde, S. K. Puri, P. Malhotra, and V. Singh Chauhan Falcipain-1, a Plasmodium falciparum Cysteine Protease with Vaccine Potential Infect. Immun., April 1, 2007; 75(4): 2026 - 2034. [Abstract] [Full Text] [PDF]

				S. Hashmi, J. Zhang, Y. Oksov, Q. Ji, and S. Lustigman The Caenorhabditis elegans CPI-2a Cystatin-like Inhibitor Has an Essential Regulatory Role during Oogenesis and Fertilization J. Biol. Chem., September 22, 2006; 281(38): 28415 - 28429. [Abstract] [Full Text] [PDF]

				L. Ford, D. B. Guiliano, Y. Oksov, A. K. Debnath, J. Liu, S. A. Williams, M. L. Blaxter, and S. Lustigman Characterization of a Novel Filarial Serine Protease Inhibitor, Ov-SPI-1, from Onchocerca volvulus, with Potential Multifunctional Roles during Development of the Parasite J. Biol. Chem., December 9, 2005; 280(49): 40845 - 40856. [Abstract] [Full Text] [PDF]

				E. Espagne, V. Douris, G. Lalmanach, B. Provost, L. Cattolico, J. Lesobre, S. Kurata, K. Iatrou, J.-M. Drezen, and E. Huguet A Virus Essential for Insect Host-Parasite Interactions Encodes Cystatins J. Virol., August 1, 2005; 79(15): 9765 - 9776. [Abstract] [Full Text] [PDF]

				J.-D. LEE and C.-M. YEN PROTEASE SECRETED BY THE INFECTIVE LARVAE OF ANGIOSTRONGYLUS CANTONENSIS AND ITS ROLE IN THE PENETRATION OF MOUSE INTESTINE Am J Trop Med Hyg, June 1, 2005; 72(6): 831 - 836. [Abstract] [Full Text] [PDF]

				S. Hashmi, J. Zhang, Y. Oksov, and S. Lustigman The Caenorhabditis elegans Cathepsin Z-like Cysteine Protease, Ce-CPZ-1, Has a Multifunctional Role during the Worms' Development J. Biol. Chem., February 13, 2004; 279(7): 6035 - 6045. [Abstract] [Full Text] [PDF]

				J. A. Ligas, L. A. Kerepesi, A. M. Galioto, S. Lustigman, T. J. Nolan, G. A. Schad, and D. Abraham Specificity and Mechanism of Immunoglobulin M (IgM)- and IgG-Dependent Protective Immunity to Larval Strongyloides stercoralis in Mice Infect. Immun., December 1, 2003; 71(12): 6835 - 6843. [Abstract] [Full Text] [PDF]

				S. Hashmi, C. Britton, J. Liu, D. B. Guiliano, Y. Oksov, and S. Lustigman Cathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans J. Biol. Chem., January 25, 2002; 277(5): 3477 - 3486. [Abstract] [Full Text] [PDF]

				D. Abraham, O. Leon, S. Leon, and S. Lustigman Development of a Recombinant Antigen Vaccine against Infection with the Filarial Worm Onchocerca volvulus Infect. Immun., January 1, 2001; 69(1): 262 - 270. [Abstract] [Full Text] [PDF]

				M. Lizotte-Waniewski, W. Tawe, D. B. Guiliano, W. Lu, J. Liu, S. A. Williams, and S. Lustigman Identification of Potential Vaccine and Drug Target Candidates by Expressed Sequence Tag Analysis and Immunoscreening of Onchocerca volvulus Larval cDNA Libraries Infect. Immun., June 1, 2000; 68(6): 3491 - 3501. [Abstract] [Full Text] [PDF]

				K. K. A. Tetteh, A. Loukas, C. Tripp, and R. M. Maizels Identification of Abundantly Expressed Novel and Conserved Genes from the Infective Larval Stage of Toxocara canis by an Expressed Sequence Tag Strategy Infect. Immun., September 1, 1999; 67(9): 4771 - 4779. [Abstract] [Full Text] [PDF]