Journal of Biological Chemistry
Volume 284, Issue 15, 10 April 2009, Pages 10160-10173
Protein Synthesis, Post-Translational Modification, and DegradationERp57 Does Not Require Interactions with Calnexin and Calreticulin to Promote Assembly of Class I Histocompatibility Molecules, and It Enhances Peptide Loading Independently of Its Redox Activity*
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The abbreviations used are: MHC, major histocompatibility complex; β2m, β2-microglobulin; Cnx, calnexin; Crt, calreticulin; CTL, cytotoxic T lymphocytes; DMEM, Dulbecco’s modified Eagle’s medium; DTT, dithiothreitol; ER, endoplasmic reticulum; H chain, heavy chain; mAb, monoclonal antibody; NEM, N-ethylmaleimide; PDI, protein-disulfide isomerase; PLC, peptide loading complex; siRNA, small interfering RNA; RNAi, RNA interference; HA, hemagglutinin; Ab, antibody; PBS, phosphate-buffered saline; MES, 4-morpholineethanesulfonic acid.
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M. Häuptle, E.-M. Frickel, and L. Ellgaard, unpublished data.
© 2009 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.