Journal of Biological Chemistry
Volume 281, Issue 7, 17 February 2006, Pages 4094-4099
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Molecular Basis of Cell and Developmental Biology
Role of the Kinesin-2 Family Protein, KIF3, during Mitosis*

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During mitosis, kinesin and dynein motor proteins play critical roles in the equal segregation of chromosomes between two daughter cells. Kinesin-2 is composed of two microtubule-based motor subunits, KIF3A/3B, and a kinesin-associated protein known as KAP3, which links KIF3A/3B to cargo that is carried to cellular organelles along microtubules in interphase cells. We have shown here that the kinesin-2 complex is localized with components of the mitotic apparatus such as spindle microtubules and centrosomes. Furthermore, we found that expression of a mutant KIF3B, which is able to associate with KIF3A but not KAP3 in NIH3T3 cells, caused chromosomal aneuploidy and abnormal spindle formation. Our data suggested that the kinesin-2 complex plays an important role not only in interphase but also in mitosis.

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This work was supported by grants-in-aid for scientific research on priority areas and the Organization for Pharmaceutical Safety and Research. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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Present Address: New Product Research Laboratories III, Daiichi Pharmaceutical Co., Ltd., Tokyo R&D Center, Tokyo 134-0081, Japan.