JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M213265200 on January 28, 2003

J. Biol. Chem., Vol. 278, Issue 16, 14066-14073, April 18, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/16/14066    most recent
M213265200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hino, S.-i.
Right arrow Articles by Kikuchi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hino, S.-i.
Right arrow Articles by Kikuchi, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Casein Kinase Iepsilon Enhances the Binding of Dvl-1 to Frat-1 and Is Essential for Wnt-3a-induced Accumulation of beta -Catenin*

Shin-ichiro HinoDagger , Tatsuo Michiue§, Makoto Asashima§, and Akira KikuchiDagger

From the Dagger  Department of Biochemistry, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan and the § Sorst Project and Department of Life Science (Biology), University of Tokyo, 3-8-1, Komaba, Meguro-ku, Tokyo 153-8902, Japan

We demonstrate that Dvl-1, casein kinase Iepsilon (CKIepsilon ), and Frat-1 activate the Wnt signaling pathway cooperatively. The amino acid region 228-250 of Dvl-1 was necessary for its binding to Frat-1, and the interaction of Dvl-1 with Frat-1 was enhanced by CKIepsilon . Coexpression of Dvl-1 and Frat-1 caused accumulation of beta -catenin synergistically in L cells. Both proteins also activated the transcriptional activity of T-cell factor-4 (Tcf-4) synergistically in human embryonic kidney 293 cells, but coexpression of Dvl-1-(Delta 228-250), which lacks the amino acid region 228-250 from Dvl-1, and Frat-1 did not. Dvl-1, but not Dvl-1-(Delta 228-250), acted synergistically with CKIepsilon to activate Tcf-4. Depletion of CKIepsilon by double-stranded RNA interference in HeLa S3 cells led to the inhibition of Wnt-3a-induced phosphorylation of Dvl and the binding of Dvl-1 to Frat-1. Furthermore, depletion of CKIepsilon reduced the Wnt-3a-induced accumulation of beta -catenin, although it did not affect the basal level of beta -catenin. These results indicate that CKIepsilon -dependent phosphorylation of Dvl enhances the formation of a complex of Dvl-1 with Frat-1 and that this complex leads to the activation of the Wnt signaling pathway.

* This work was supported by Grants-in-Aid for Scientific Research and for Scientific Research on priority areas from the Ministry of Education, Science, and Culture, Japan (2001, 2002).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 81-82-257-5130; Fax: 81-82-257-5134; E-mail: akikuchi@hiroshima-u.ac.jp.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Izumi, K. Fumoto, S. Izumi, and A. Kikuchi
GSK-3{beta} Regulates Proper Mitotic Spindle Formation in Cooperation with a Component of the {gamma}-Tubulin Ring Complex, GCP5
J. Biol. Chem., May 9, 2008; 283(19): 12981 - 12991.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. A. Martino, G. Y. Oudit, A. M. Herzenberg, N. Tata, M. M. Koletar, G. M. Kabir, D. D. Belsham, P. H. Backx, M. R. Ralph, and M. J. Sole
Circadian rhythm disorganization produces profound cardiovascular and renal disease in hamsters
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1675 - R1683.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
M. Campagna, M. Budini, F. Arnoldi, U. Desselberger, J. E. Allende, and O. R. Burrone
Impaired hyperphosphorylation of rotavirus NSP5 in cells depleted of casein kinase 1{alpha} is associated with the formation of viroplasms with altered morphology and a moderate decrease in virus replication
J. Gen. Virol., October 1, 2007; 88(10): 2800 - 2810.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Schwarz-Romond, C. Metcalfe, and M. Bienz
Dynamic recruitment of axin by Dishevelled protein assemblies
J. Cell Sci., July 15, 2007; 120(14): 2402 - 2412.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Bryja, G. Schulte, N. Rawal, A. Grahn, and E. Arenas
Wnt-5a induces Dishevelled phosphorylation and dopaminergic differentiation via a CK1-dependent mechanism
J. Cell Sci., February 15, 2007; 120(4): 586 - 595.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
S. Kishida, K. Hamao, M. Inoue, M. Hasegawa, Y. Matsuura, K. Mikoshiba, M. Fukuda, and A. Kikuchi
Dvl regulates endo- and exocytotic processes through binding to synaptotagmin.
Genes Cells, January 1, 2007; 12(1): 49 - 61.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Quintavalle, S. Sambucini, C. Di Pietro, R. De Francesco, and P. Neddermann
The {alpha} Isoform of Protein Kinase CKI Is Responsible for Hepatitis C Virus NS5A Hyperphosphorylation
J. Virol., November 15, 2006; 80(22): 11305 - 11312.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Gustafson and U. Smith
Cytokines Promote Wnt Signaling and Inflammation and Impair the Normal Differentiation and Lipid Accumulation in 3T3-L1 Preadipocytes
J. Biol. Chem., April 7, 2006; 281(14): 9507 - 9516.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
H. Jian, X. Shen, I. Liu, M. Semenov, X. He, and X.-F. Wang
Smad3-dependent nuclear translocation of beta-catenin is required for TGF-beta1-induced proliferation of bone marrow-derived adult human mesenchymal stem cells.
Genes & Dev., March 15, 2006; 20(6): 666 - 674.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. A. Price
CKI, there's more than one: casein kinase I family members in Wnt and Hedgehog signaling.
Genes & Dev., February 15, 2006; 20(4): 399 - 410.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Kobayashi, S.-i. Hino, N. Oue, T. Asahara, M. Zollo, W. Yasui, and A. Kikuchi
Glycogen Synthase Kinase 3 and h-prune Regulate Cell Migration by Modulating Focal Adhesions
Mol. Cell. Biol., February 1, 2006; 26(3): 898 - 911.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. M. Cadigan and Y. I. Liu
Wnt signaling: complexity at the surface
J. Cell Sci., February 1, 2006; 119(3): 395 - 402.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S.-i. Hino, C. Tanji, K. I. Nakayama, and A. Kikuchi
Phosphorylation of {beta}-Catenin by Cyclic AMP-Dependent Protein Kinase Stabilizes {beta}-Catenin through Inhibition of Its Ubiquitination
Mol. Cell. Biol., October 15, 2005; 25(20): 9063 - 9072.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Ihara, H. Yamamoto, and A. Kikuchi
SUMO-1 Modification of PIASy, an E3 Ligase, Is Necessary for PIASy-Dependent Activation of Tcf-4
Mol. Cell. Biol., May 1, 2005; 25(9): 3506 - 3518.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Luo, H. Zou, L. Jin, S. Lin, Q. Li, Z. Ye, H. Rui, and S.-C. Lin
Axin Contains Three Separable Domains That Confer Intramolecular, Homodimeric, and Heterodimeric Interactions Involved in Distinct Functions
J. Biol. Chem., February 11, 2005; 280(6): 5054 - 5060.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. S. Waxman, A. M. Hocking, C. L. Stoick, and R. T. Moon
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes
Development, December 1, 2004; 131(23): 5909 - 5921.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. S. Waddell, N. T. Liberati, X. Guo, J. P. Frederick, and X.-F. Wang
Casein Kinase I{epsilon} Plays a Functional Role in the Transforming Growth Factor-{beta} Signaling Pathway
J. Biol. Chem., July 9, 2004; 279(28): 29236 - 29246.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. van Amerongen, H. van der Gulden, F. Bleeker, J. Jonkers, and A. Berns
Characterization and Functional Analysis of the Murine Frat2 Gene
J. Biol. Chem., June 25, 2004; 279(26): 26967 - 26974.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. E. Weitzel, M. R. Illies, C. A. Byrum, R. Xu, A. H. Wikramanayake, and C. A. Ettensohn
Differential stability of {beta}-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled
Development, June 15, 2004; 131(12): 2947 - 2956.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Kishida, H. Yamamoto, and A. Kikuchi
Wnt-3a and Dvl Induce Neurite Retraction by Activating Rho-Associated Kinase
Mol. Cell. Biol., May 15, 2004; 24(10): 4487 - 4501.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Swiatek, I-C. Tsai, L. Klimowski, A. Pepler, J. Barnette, H. J. Yost, and D. M. Virshup
Regulation of Casein Kinase I{epsilon} Activity by Wnt Signaling
J. Biol. Chem., March 26, 2004; 279(13): 13011 - 13017.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. Cong, L. Schweizer, and H. Varmus
Casein Kinase I{varepsilon} Modulates the Signaling Specificities of Dishevelled
Mol. Cell. Biol., March 1, 2004; 24(5): 2000 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Matsubayashi, S. Sese, J.-S. Lee, T. Shirakawa, T. Iwatsubo, T. Tomita, and S.-i. Yanagawa
Biochemical Characterization of the Drosophila Wingless Signaling Pathway Based on RNA Interference
Mol. Cell. Biol., March 1, 2004; 24(5): 2012 - 2024.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Weaver, G. H. Farr III, W. Pan, B. A. Rowning, J. Wang, J. Mao, D. Wu, L. Li, C. A. Larabell, and D. Kimelman
GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs
Development, November 15, 2003; 130(22): 5425 - 5436.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Marin, V. H. Bustos, L. Cesaro, F. Meggio, M. A. Pagano, M. Antonelli, C. C. Allende, L. A. Pinna, and J. E. Allende
Inaugural Article: A noncanonical sequence phosphorylated by casein kinase 1 in {beta}-catenin may play a role in casein kinase 1 targeting of important signaling proteins
PNAS, September 2, 2003; 100(18): 10193 - 10200.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.