JBC Biosymposia, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M203361200 on May 28, 2002

J. Biol. Chem., Vol. 277, Issue 33, 29484-29489, August 16, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/33/29484    most recent
M203361200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Farzan, M.
Right arrow Articles by Choe, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Farzan, M.
Right arrow Articles by Choe, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Role of Post-translational Modifications of the CXCR4 Amino Terminus in Stromal-derived Factor 1alpha Association and HIV-1 Entry*

Michael FarzanDagger , Gregory J. BabcockDagger , Natalya Vasilieva§, Paulette L. WrightDagger , Enko KiprilovDagger , Tajib MirzabekovDagger , and Hyeryun Choe§

From the Dagger  Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Department of Pathology, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115 and § Perlmutter Laboratory, Children's Hospital, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115

The chemokine receptor CXCR4 plays critical roles in development, immune function, and human immunodeficiency virus type 1 (HIV-1) entry. Here we demonstrate that, like the CC-chemokine receptors CCR5 and CCR2b, CXCR4 is posttranslationally modified by sulfation of its amino-terminal tyrosines. The sulfate group at tyrosine 21 contributes substantially to the ability of CXCR4 to bind its ligand, stromal derived factor 1alpha . Tyrosine sulfation plays a less significant role in CXCR4-dependent HIV-1 entry than in CCR5-dependent HIV-1 entry. In some cell lines, CXCR4 is efficiently modified by a chondroitin sulfate chain at serine 18, but neither HIV-1 entry nor stromal derived factor 1alpha binding was affected by loss of this glycosaminoglycan. These data demonstrate a functional role for tyrosine sulfate in the CXC-chemokine receptor family and underscore a general difference in HIV-1 utilization of CCR5 and CXCR4.

* This work was supported by National Institutes of Health Grants AI48425 (to M. F.) and AI43891 (to H. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Perlmutter Laboratory, 300 Longwood Ave., Children's Hospital, Boston, MA 02115. E-mail: hchoe@mbcrr.harvard.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Sci SignalHome page
C. T. Veldkamp, C. Seibert, F. C. Peterson, N. B. De la Cruz, J. C. Haugner III, H. Basnet, T. P. Sakmar, and B. F. Volkman
Structural Basis of CXCR4 Sulfotyrosine Recognition by the Chemokine SDF-1/CXCL12
Sci. Signal., September 16, 2008; 1(37): ra4 - ra4.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Liu, S. Louie, W. Hsu, K. M. Yu, H. B. Nicholas Jr., and G. L. Rosenquist
Tyrosine Sulfation Is Prevalent in Human Chemokine Receptors Important in Lung Disease
Am. J. Respir. Cell Mol. Biol., June 1, 2008; 38(6): 738 - 743.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
F. Reggeti, C. Ackerley, and D. Bienzle
CD134 and CXCR4 expression corresponds to feline immunodeficiency virus infection of lymphocytes, macrophages and dendritic cells
J. Gen. Virol., January 1, 2008; 89(1): 277 - 287.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Xu, J. Sui, H. Tao, Q. Zhu, and W. A. Marasco
Human Anti-CXCR4 Antibodies Undergo VH Replacement, Exhibit Functional V-Region Sulfation, and Define CXCR4 Antigenic Heterogeneity
J. Immunol., August 15, 2007; 179(4): 2408 - 2418.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. W. Kehoe, N. Velappan, M. Walbolt, J. Rasmussen, D. King, J. Lou, K. Knopp, P. Pavlik, J. D. Marks, C. R. Bertozzi, et al.
Using Phage Display to Select Antibodies Recognizing Post-translational Modifications Independently of Sequence Context
Mol. Cell. Proteomics, December 1, 2006; 5(12): 2350 - 2363.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Dorfman, M. J. Moore, A. C. Guth, H. Choe, and M. Farzan
A Tyrosine-sulfated Peptide Derived from the Heavy-chain CDR3 Region of an HIV-1-neutralizing Antibody Binds gp120 and Inhibits HIV-1 Infection
J. Biol. Chem., September 29, 2006; 281(39): 28529 - 28535.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. A. Colvin, G. S. V. Campanella, L. A. Manice, and A. D. Luster
CXCR3 Requires Tyrosine Sulfation for Ligand Binding and a Second Extracellular Loop Arginine Residue for Ligand-Induced Chemotaxis
Mol. Cell. Biol., August 1, 2006; 26(15): 5838 - 5849.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Zaitseva, T. Romantseva, J. Manischewitz, J. Wang, D. Goucher, and H. Golding
Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association
J. Leukoc. Biol., December 1, 2005; 78(6): 1306 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Kouroumalis, R. J. Nibbs, H. Aptel, K. L. Wright, G. Kolios, and S. G. Ward
The Chemokines CXCL9, CXCL10, and CXCL11 Differentially Stimulate G{alpha}i-Independent Signaling and Actin Responses in Human Intestinal Myofibroblasts
J. Immunol., October 15, 2005; 175(8): 5403 - 5411.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
M. Kucia, R. Reca, K. Miekus, J. Wanzeck, W. Wojakowski, A. Janowska-Wieczorek, J. Ratajczak, and M. Z. Ratajczak
Trafficking of Normal Stem Cells and Metastasis of Cancer Stem Cells Involve Similar Mechanisms: Pivotal Role of the SDF-1-CXCR4 Axis
Stem Cells, August 1, 2005; 23(7): 879 - 894.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Hirata, Y. Furukawa, B.-G. Yang, K. Hieshima, M. Fukuda, R. Kannagi, O. Yoshie, and M. Miyasaka
Human P-selectin Glycoprotein Ligand-1 (PSGL-1) Interacts with the Skin-associated Chemokine CCL27 via Sulfated Tyrosines at the PSGL-1 Amino Terminus
J. Biol. Chem., December 10, 2004; 279(50): 51775 - 51782.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Bloushtain, U. Qimron, A. Bar-Ilan, O. Hershkovitz, R. Gazit, E. Fima{dagger}, M. Korc, I. Vlodavsky, N. V. Bovin, and A. Porgador
Membrane-Associated Heparan Sulfate Proteoglycans Are Involved in the Recognition of Cellular Targets by NKp30 and NKp46
J. Immunol., August 15, 2004; 173(4): 2392 - 2401.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Gutierrez, L. Kremer, A. Zaballos, I. Goya, C. Martinez-A., and G. Marquez
Analysis of Post-translational CCR8 Modifications and Their Influence on Receptor Activity
J. Biol. Chem., April 9, 2004; 279(15): 14726 - 14733.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. E. Kuhmann, P. Pugach, K. J. Kunstman, J. Taylor, R. L. Stanfield, A. Snyder, J. M. Strizki, J. Riley, B. M. Baroudy, I. A. Wilson, et al.
Genetic and Phenotypic Analyses of Human Immunodeficiency Virus Type 1 Escape from a Small-Molecule CCR5 Inhibitor
J. Virol., March 15, 2004; 78(6): 2790 - 2807.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Onnerfjord, T. F. Heathfield, and D. Heinegard
Identification of Tyrosine Sulfation in Extracellular Leucine-rich Repeat Proteins Using Mass Spectrometry
J. Biol. Chem., January 2, 2004; 279(1): 26 - 33.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Gao, H. Choe, D. Bota, P. L. Wright, C. Gerard, and N. P. Gerard
Sulfation of Tyrosine 174 in the Human C3a Receptor Is Essential for Binding of C3a Anaphylatoxin
J. Biol. Chem., September 26, 2003; 278(39): 37902 - 37908.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. L. Moore
The Biology and Enzymology of Protein Tyrosine O-Sulfation
J. Biol. Chem., June 27, 2003; 278(27): 24243 - 24246.
[Full Text] [PDF]

Home page
 J. Virol.Home page
G. Lin, F. Baribaud, J. Romano, R. W. Doms, and J. A. Hoxie
Identification of gp120 Binding Sites on CXCR4 by Using CD4-Independent Human Immunodeficiency Virus Type 2 Env Proteins
J. Virol., December 20, 2002; 77(2): 931 - 942.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Farzan, S. Chung, W. Li, N. Vasilieva, P. L. Wright, C. E. Schnitzler, R. J. Marchione, C. Gerard, N. P. Gerard, J. Sodroski, et al.
Tyrosine-sulfated Peptides Functionally Reconstitute a CCR5 Variant Lacking a Critical Amino-terminal Region
J. Biol. Chem., October 18, 2002; 277(43): 40397 - 40402.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.