Journal of Biological Chemistry
Volume 271, Issue 49, 6 December 1996, Pages 31679-31687
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Nucleic Acids, Protein Synthesis, and Molecular Genetics
The Far Upstream Element-binding Proteins Comprise an Ancient Family of Single-strand DNA-binding Transactivators*

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The cloning and expression of two new human cDNAs encoding proteins highly related to the far upstream element-binding protein (FBP) are described. FBP, FBP2, and FBP3 comprise a family of single-strand DNA- binding proteins that possess all of the general features of more conventional transcription factors. The FBPs each bind sequence specifically to only one strand of the far upstream element (FUSE; originally identified upstream of c-myc), and each possesses potent activation domains when fused to the GAL4 DNA-binding domain and assayed by transient transfection. Typical of transcription factors, the proteins are most highly related in their central, DNA-binding domains, but extensive homology is also shared within the tyrosine-rich, carboxyl-terminal activation domains. Comparison with GenBank sequences revealed a fourth FBP family member encoded by Caenorhabditis elegans chromosome III, illustrating the high degree of homology in this evolutionarily ancient and conserved family.

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*

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) U69126 (FBP2) and U69127 (FBP3).

Present address: Dept. of Cardiovascular Research, Genentech, Inc., South San Francisco, CA 94080-4990.

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Present address: Laboratory of Molecular Pharmacology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892.