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(Received for publication, April 1, 1996, and in revised form, April 26, 1996)
From the Section of Microbiology, University of California, Davis,
California 95616 and the We have explored the phenotypic and genetic
overlap between autophagocytosis and cytoplasm to vacuole targeting in
the yeast Saccharomyces cerevisiae. Complementation
analysis was performed with mutants in each of these groups
(aut and cvt, respectively), and three
complementation groups were found to overlap. Also, most of the unique
aut mutants accumulated precursor aminopeptidase I in the
cytoplasm, while maintaining wild type kinetics and maturation of
proteins targeted to the vacuole via the secretory pathway. The
majority of the non-overlapping cvt mutants were found to
be at least partially defective in autophagy. Some mutants in each
group, however, appear to be only marginally affected in the other
phenotype, implying that these pathways only partially overlap. We
propose that import of aminopeptidase I into the vacuole shares a
number of components required for bulk autophagocytosis, but is made
specific, saturable, and constitutive by the presence of a receptor or
other interacting protein(s).
Volume 271, Number 30,
Issue of July 26, 1996
pp. 17621-17624
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
COMMUNICATION:
and
Institut für
Biochemie, Universität Stuttgart, 70569 Stuttgart,
Germany
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