Somatic Embryogenesis in the Leaf Explants of Chinese Celery

Abstract

The development of embryoids from leaf explants of Apium graveolens var. dulce cultured on Murashige and Skoog medium with 0.5 mg/l 2,4-D and 0.6 mg/l kinetin was followed. Four stages were recognized: (1) cell proliferation, (2) pro-embryoid formation, (3) torpedo embryoid formation and (4) plantlet formation. All embryoids were derived from the cortical tissues of leaf explants; no embryoids formed in the epidermal tissues.