Abstract
Downy mildew (caused by Peronospora viciae) and powdery mildew (caused by Erysiphe pisi) cause significant yield losses in field pea crops of southern Australia. The Australian Coordinated Pea Improvement Program (ACPIP) aims to select lines that are resistant to both of these pathogens. A method was developed to allow screening of early generation material for resistance against both diseases, through consecutive testing on single plants. The apical buds of plants were inoculated with conidial suspensions of P. viciae and the same plants were infected with E. pisi via airborne spores in the greenhouse. Of 88 lines tested, 25 had useful downy mildew resistance, 19 lines were resistant to powdery mildew and 14 lines displayed resistance to both pathogens. The results of the controlled environment and greenhouse trials were highly correlated with results of field screening; for downy mildew r = 0.88 (P < 0.001) and for powdery mildew r=0.72 (P < 0.001).
Similar content being viewed by others
References
Ali SM, Sharma B, Ambrose MJ (1994) Current status and future strategy in breeding pea to improve resistance to biotic and abiotic stresses. Euphytica 73, 115–126.
Biddle AJ (2001) Downy mildew. In ‘Compendium of pea diseases and pests (2nd edn)’. (Eds JM Kraft, FL Pfleger) pp. 29–30. (APS Press: St Paul, USA)
Davidson JA, Ramsey MD (2000) Pea yield decline syndrome in South Australia: the role of diseases and the impact of agronomic practices. Australian Journal of Agricultural Research 51, 347–354. doi:10.1071/AR99111
Falloon RE, McErlich AF, Scott RE (1990) Effects of powdery mildew on vining and seed yields of garden peas. In ‘Proceedings of the 43rd New Zealand Weed and Pest Control Conference’, pp. 39–42. (New Zealand Weed and Pest Control Society: Palmerston North, NZ)
Falloon RE, Viljanen-Rollinson SLH (2001) Powdery mildew. In ‘Compendium of pea diseases and pests (2nd edn)’. (Eds JM Kraft, FL Pfleger) pp. 28–29. (APS Press: St Paul, USA)
Harland SC (1948) Inheritance of immunity to mildew in Peruvian forms of Pisum sativum. Heredity 2, 263–269.
Heringa RJ, Vannorel A, Tazelaar MF (1969) Resistance to powdery mildew (Erysiphe polygoni D.C.) in peas (Pisurn sativum L.). Euphytica 18, 163–169.
Kumar H, Singh RB (1981) Genetic analysis of adult plant resistance to powdery mildew in pea (Pisum sativum L.). Euphytica 30, 147–151.
Lewis BG, Matthews KP (1985) World germplasm of Pisum sativum: Could it be used more effectively to produce healthy crops? In ‘The pea crop’. (Eds PD Hebblethwaite, MC Heath, TCK Dawkins) pp. 215–229. (Butterworths: London, UK)
Matthews P, Dow KP (1972) ‘Pea pathogens.’ John Innes Institute, 63rd Annual Report, pp. 38–39. (John Innes Institute: Norwich, UK)
Munjal RL, Chenulu VV, Hora TS (1963) Assessment of losses due to powdery mildew (Erysiphe polygoni) on pea. Indian Phytopathology 19, 260–267.
Peck DM, McDonald GK (2001) Survey of field pea production practices in South Australia. In ‘Proceedings of 10th Australian agronomy conference’. 29 January–1 February 2003, Hobart, Tasmania. Available online at www.regional.org.au/au/asa/2001/ (verified 27 June 2004)
Singh UP, Mishra GD (1992) Effect of powdery mildew (Erysiphe pisi) on nodulation and nitrogenase activity in pea (Pisum sativum). Plant Pathology 41, 262–264.
Sivapalan A (1993) Effects of impacting rain drops on the growth and development of powdery mildew fungi. Plant Pathology 42, 256–263.
Taylor PN (1986) Resistance of Pisum sativum to Peronospora pisi. PhD Thesis, University of East Anglia, UK.
Taylor PN, Lewis BG, Matthews P (1989) Pathotypes of Peronospora viciae in Britain. Journal of Phytopathology 127, 100–106.
Tiwari KR, Penner GA, Warkentin TD (1997a) Inheritance of powdery mildew resistance in pea. Canadian Journal of Plant Science 77, 307–310.
Tiwari KR, Penner GA, Warkentin TD, Rashid KY (1997) Pathogenic variation in Erysiphe pisi, the causal organism of powdery mildew of pea. Canadian Journal of Plant Pathology 19, 267–271.
Van der Gaag DJ, Frinking HD (1997) Survival characteristics of oospore populations of Peronospora viciae f. sp. pisi in soil. Plant Pathology 46, 978–988. doi:10.1046/J.1365-3059.1997.D01-89.X
Viljanen-Rollinson SLH, Gaunt RE, Frampton CMA, Falloon RE, McNeil DL (1998a) Components of quantitative resistance to powdery mildew (Erysiphe pisi) in pea (Pisum sativum). Plant Pathology 47, 137–147. doi: 10.1046/J.1365-3059.1998.00217.X
Viljanen-Rollinson SLH, Frampton CMA, Gaunt RE, Falloon RE, McNeil DL (1998b) Spatial and temporal spread of powdery mildew (Erysiphe pisi) in peas (Pisum sativum) varying in quantitative resistance. Plant Pathology 47, 148–156. doi: 10.1046/J. 1365-3059.1998.0023 l.X
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Davidson, J.A., Krysinska-Kaczmarek, M., Kimber, R.B.E. et al. Screening field pea germplasm for resistance to downy mildew (Peronospora viciae) and powdery mildew (Erysiphepisi). Australasian Plant Pathology 33, 413–417 (2004). https://doi.org/10.1071/AP04040
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1071/AP04040