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Effect of acibenzolar-S-methyl and Saccharomyces cerevisiae on the activation of Eucalyptus defences against rust

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Abstract

Tree defence mechanisms against the fungus Puccinia psidii were examined by comparing the activities of defence-related enzymes (chitinase, peroxidase and phenylalanine ammonia-lyase) of two Eucalyptus grandis × E. urophylla (urograndis) hybrids, previously classified as either susceptible to rust (VR hybrid) or moderately resistant to rust (C0 hybrid). Furthermore, the potential of disease control by artificial activation of host defences using either acibenzolar-S-methyl (ASM) or Saccharomyces cerevisiae extract was also investigated. Greenhouse inoculation trials revealed that the C0 hybrid had lower disease severity than the VR hybrid but following foliar applications of either ASM or S. cerevisiae extract treatment, disease severity (evaluated at 15 days after inoculation) was reduced in both hybrids. This enhanced resistance was associated with the induction of a hypersensitive reaction which appeared to be effective in controlling rust in both clones. The activity of all enzymes differed between clones and inducer treatment. The role of the defence-related enzymes in imparting resistance to eucalypt hybrids against rust is discussed.

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Authors and Affiliations

  1. Departamento de Produção Vegetal, Setor de Defesa Fitossanitária, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista ‘Julio de Mesquita Filho’, CEP 18603-970, CP 237, Botucatu, SP, Brasil

    Leonardo Pires Boava & Edson Luiz Furtado

  2. Universidade Federal do Pampa, CEP 97650-000, Itaqui, RS, Brasil

    Odair J. Kuhn

  3. Setor de Fitopatologia, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo, CEP 13418-900, Piracicaba, SP, Brasil

    Sérgio Florentino Pascholati

  4. Departamento de Fitotecnia, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, CEP 88040-900, Florianópolis, SC, Brasil

    Robson M. Di Piero

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  1. Leonardo Pires Boava
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  2. Odair J. Kuhn
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  3. Sérgio Florentino Pascholati
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  4. Robson M. Di Piero
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  5. Edson Luiz Furtado
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Correspondence to Leonardo Pires Boava.

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Boava, L.P., Kuhn, O.J., Pascholati, S.F. et al. Effect of acibenzolar-S-methyl and Saccharomyces cerevisiae on the activation of Eucalyptus defences against rust. Australasian Plant Pathology 38, 594–602 (2009). https://doi.org/10.1071/AP09045

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