Horm Metab Res 2008; 40(11): 806-812
DOI: 10.1055/s-2008-1081503
Humans, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Relation of Plasma Obestatin Levels to BMI, Gender, Age and Insulin

F. Lippl 1 , J. Erdmann 2 , N. Lichter 1 , S. Tholl 2 , S. Wagenpfeil 3 , O. Adam 4 , V. Schusdziarra 2
  • 1Division of Gastroenterology, Department of Internal Medicine, University Hospital of the Ludwig-Maximilians-University, Munich, Germany
  • 2Else-Kröner-Fresenius Center of Nutritional Medicine, Technical University of Munich, Munich, Germany
  • 3Department of Medical Statistics and Epidemiology, Technical University of Munich, Munich, Germany
  • 4Walther-Straub Institute, Ludwig-Maximilians-University, Munich, Germany
Further Information

Publication History

received 16.10.2007

accepted 17.04.2008

Publication Date:
11 July 2008 (online)

Abstract

Obestatin is supposed to be involved in nutrient homeostasis. Therefore, basal plasma obestatin levels were investigated in 321 normal weight and obese subjects in relation to body mass index, gender, age, insulin concentrations, and type 2 diabetes mellitus. Additionally, postprandial obestatin levels were determined in 20 normal weight subjects. Basal obestatin levels in females were higher compared to males (193.6±5.8 vs. 140.6±5.1 pg/ml). Obestatin levels correlated inversely and significantly with body mass index (f: r=−0.632, p<0.001; m: r=−0.487, p<0.001) and basal insulin levels (f: r=−0.536, p<0.001; m: r=−0.320, p=0.008) in females and males. However, in a multiple regression analysis as well as in a matched comparison of a low and high insulin group no significant relationship between insulin and obestatin levels was observed in nondiabetics. On the other hand, inclusion of type 2 diabetics with higher insulin levels resulted in a significant inverse correlation. Obestatin levels were independent of age in both sexes. In patients with type 2 diabetes mellitus basal obestatin levels were not different compared to nondiabetic subjects when matched for gender, body mass index, and insulin. In normal weight subjects, postprandial obestatin levels showed a significant decrease between 60 and 90 minutes rising to basal levels thereafter. The present data demonstrate a relation of plasma obestatin levels to body weight, gender and food intake, but not to age. The inverse relationship with insulin might depend on the level of hyperinsulinemia. The present data are compatible with a potential role of obestatin in nutrient regulation.

References

  • 1 Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake.  Science. 2005;  310 996-999
  • 2 Dun SL, Brailoiu GC, Brailoiu E, Yang J, Chang JK, Dun NJ. Distribution and biological activity of obestatin in the rat.  J Endocrinol. 2006;  191 481-489
  • 3 Lauwers E, Landuyt B, Arckens L, Schoofs L, Luyten W. Obestatin does not activate orphan G protein-coupled receptor GPR39.  Biochem Biophys Res Commun. 2006;  351 21-25
  • 4 Holst B, Egerod KL, Schild E, Vickers SP, Cheetham S, Gerlach LO, Storjohann L, Stidsen CE, Jones R, Beck-Sickinger AG, Schwartz TW. GPR39 signaling is stimulated by zinc ions but not by obestatin.  Endocrinology. 2007;  148 13-20
  • 5 Tremblay F, Perreault M, Klaman LD, Tobin JF, Smith E, Gimeno RE. Normal food intake and body weight in mice lacking the G protein-coupled receptor GPR39.  Endocrinology. 2006;  501-506
  • 6 Sun Y, Ahmed S, Smith RG. Deletion of ghrelin impairs neither growth nor appetite.  Mol Cell Biol. 2003;  23 7973-7981
  • 7 Wortley KE, Anderson KD, Garcia K, Murray JD, Malinova L, Liu R, Moncrieffe M, Thabet K, Cox HJ, Yancopoulos GD, Wiegand SJ, Sleeman MW. Genetic deletion of ghrelin does not decrease food intake but influences metabolic fuel preference.  Proc Natl Acad Sci USA. 2004;  101 8227-8232
  • 8 Zizzari P, Longchamps R, Epelbaum J, Bluet-Pajot MT. Obestatin partially affects ghrelin stimulation of food intake and growth hormone secretion in rodents.  Endocrinology. 2007;  1 1648-1653
  • 9 Carlini VP, Schioth HB, Debarioglio SR. Obestatin improves memory performance and causes anxiolytic effects in rats.  Biochem Biophys Res Commun. 2007;  352 907-912
  • 10 Nogueiras R, Pfluger P, Tovar S, Arnold M, Mitchell S, Morris A, Perez-Tilve D, Vazquez MJ, Wiedmer P, Castaneda TR, DiMarchi R, Tschop M, Schurmann A, Joost HG, Williams LM, Langhans W, Dieguez C. Effects of obestatin on energy balance and growth hormone secretion in rodents.  Endocrinology. 2007;  148 21-26
  • 11 Seoane LM, Al Massadi O, Pazos Y, Pagotto U, Casanueva FF. Central obestatin administration does not modify either spontaneous or ghrelin-induced food intake in rats.  J Endocrinol Invest. 2006;  29 RC13-RC15
  • 12 Gourcerol G, Tache Y. Obestatin – a ghrelin-associated peptide that does not hold its promise to suppress food intake and motility.  Neurogastroenterol Motil. 2007;  19 161-165
  • 13 Smet B de, Thijs T, Peeters TL, Depoortere I. Effect of peripheral obestatin on gastric emptying and intestinal contractility in rodents.  Neurogastroenterol Motil. 2007;  19 211-217
  • 14 Haider DG, Schindler K, Prager G, Bohdjalian A, Luger A, Wolzt M, Ludvik B. Serum retinol-binding protein-4 is reduced after weight loss in morbidly obese subjects.  J Clin Endocrinol Metabol. 2006;  92 1168-1171
  • 15 Guo ZF, Zheng X, Qin YW, Hu JQ, Chen SP, Zhang Z. Circulating preprandial ghrelin to obestatin ratio is increased in human obesity.  J Clin Endocrinol Metabol. 2007;  92 1875-1880
  • 16 Huda MS, Durham BH, Wong SP, Deepak D, Kerrigan D, MacCulloch P, Ranganath L, Pinkney J, Wilding JP. Plasma obestatin levels are lower in obese and post-gastrectomy subjects, but do not change in response to a meal.  Int J Obes (Lond). 2007;  32 129-135
  • 17 Harada T, Nakahara T, Yasuhara D, Kojima S, Sagiyama KI, Amitani H, Laviano A, Naruo T, Inui A. Obestatin, acyl ghrelin, and des-acyl ghrelin responses to an oral glucose tolerance test in the restricting type of anorexia nervosa.  Biol Psychiatry. 2007;  63 245-247
  • 18 Reinehr T, Sousa G de, Roth CL. Obestatin and ghrelin levels in obese children and adolescents before and after reduction of overweight.  Clin Endocrinol (Oxf). 2007;  68 304-310
  • 19 Butler MG, Bittel DC. Plasma obestatin and ghrelin levels in subjects with Prader-Willi syndrome.  Am J Med Genet A. 2007;  143 415-421
  • 20 Vicennati V, Genghini S, Iasio R De, Pasqui F, Pagotto U, Pasquali R. Circulating obestatin levels and the ghrelin/obestatin ratio in obese women.  Eur J Endocrinol. 2007;  157 295-301
  • 21 Anderwald-Stadler M, Krebs M, Promintzer M, Mandl M, Bischof MG, Nowotny P, Kaestenbauer T, Luger A, Prager R, Anderwald C. Plasma obestatin is lower at fasting and not suppressed by insulin in insulin-resistant humans.  Am J Physiol Endocrinol Metab. 2007;  293 1393-1398
  • 22 Qi X, Li L, Yang G, Liu J, Li K, Tang Y, Liou H, Boden G. Circulating obestatin levels in normal subjects and in patients with impaired glucose regulation and type 2 diabetes mellitus.  Clin Endocrinol (Oxf). 2007;  66 593-597
  • 23 Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man.  Diabetologia. 1985;  28 412-419
  • 24 Krzyzanowska-Swiniarska B, Kempa A, Miazgowski T, Pilarska K. Serum acylated ghrelin, adiponectin and leptin levels in normal-weight and obese premenopausal women.  Horm Metab Res. 2007;  39 835-839
  • 25 Owecki M, Miczke A, Pupek-Musialik D, Bryl W, Cymerys M, Nikisch E, Sowinski J. Circulating serum adiponectin concentrations do not differ between obese and non-obese caucasians and are unrelated to insulin sensitivity.  Horm Metab Res. 2007;  39 25-30
  • 26 Erdmann J, Lippl F, Wagenpfeil S, Schusdziarra V. Differential association of Basal and postprandial plasma ghrelin with leptin, insulin, and type 2 diabetes.  Diabetes. 2005;  54 1371-1378

Correspondence

Dr. F. Lippl

Division of Gastroenterology Department of Internal Medicine

University Hospital of the Ludwig-Maximilians-University

Ziemssenstr. 1

80336 Munich

Germany

Phone: +49/89/5160 21 11

Fax: +49/89/5160 23 61

Email: florian.lippl@med.uni-muenchen.de

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