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DOI: 10.1055/s-2007-978627
© Georg Thieme Verlag Stuttgart · New York
Effects of Early Protein Restriction and Adult Obesity on Rat Pancreatic Hormone Content and Glucose Tolerance
Publication History
1999
2000
Publication Date:
19 April 2007 (online)
Rats were fed a diet containing either 20% (“control”) or 8% (“reduced-protein”) protein throughout pregnancy and lactation. Their female offspring were weaned onto the same respective diets. At 63 days of age one set of control and reduced-protein rats (n = 16 per group) underwent intraperitoneal glucose tolerance tests and one week later were killed and their pancreatic hormones extracted and measured. The reduced protein rats had better glucose tolerance (p < 0.001) and lower pancreatic insulin (p < 0.01) and amylin (p < 0.01) contents. Further sets of control and reduced-protein rats were then fed either chow or a cafeteria-style diet (n = 16 in each of the four groups). These rats underwent intraperitoneal glucose tolerance tests at 133 days of age, which showed the cafeteria-fed animals to have a worse glucose tolerance than the chow-fed animals irrespective of previous diet exposure (p < 0.0001). One week later reduced-protein rats still had lower pancreatic insulin contents (p < 0.05) (and a trend for lower amylin contents), but also had increased pancreatic glucagon contents (p < 0.05). There were no detectable differences in pancreatic somatostatin-like immunoreactivity or pancreatic polypeptide contents. These results are consistent with pancreatic β- and α-cells being selectively susceptible to effects associated with early dietary protein restriction.
Key words
Diabetes Mellitus - Insulin - Amylin - Glucagon - Somatostatin - Pancreatic Polypeptide