Roles of Ginsenosides on Morphine-Induced Hyperactivity and Rewarding Effect in Mice

 

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Abstract

Ginsenosides, the main effective components of the root of Panax ginseng, have been reported to modulate morphine action. In the present study, ginsenosides Rd, Rb2, Rg1 and Re were divided into two groups according to their effects in mice on morphine-induced hyperactivity and conditioned place preference (CPP). Ginsenosides Rd, Rb2, Rg1 had no effect on morphine-induced hyperactivity, but antagonized morphine-induced CPP. On the contrary, ginsenoside Re increased morphine-induced hyperactivity whereas it showed no effect on morphine-induced CPP. Furthermore, Re antagonized the inhibitory effect of the mixture involving Rd, Rb2 and Rg1 on the morphine action. These results suggest that ginsenosides with different structures have antagonizing properties in the regulation of morphine-induced reinforcement.

Abbreviations

CPP:conditioned place preference

NS:saline

Mor :morphine

G1111:ginsenosides Rd, Rb2, Rg1 and Re mixture at ratio of 1 : 1:1 : 1

G1112:ginsenosides Rd, Rb2, Rg1 and Re mixture at ratio of 1 : 1:1 : 2

L :low dose of ginsenosides, 0.5 × 10 ^{- 5} mol/kg

H:high dose of ginsenosides, 1 × 10 ^{- 5} mol/kg

References

• 1 Liu C X, Xiao P G. Recent advances on ginseng research in China.  J Ethnopharmacol. 1992;  36 27-38
  CrossrefPubMedGoogle Scholar
• 2 Soldati F, Sticher O. HPLC separation and quantitative determination of ginsenosides from Panax ginseng, Panax quinquefolium and from ginseng drug preparations. 2nd communication.  Planta Med. 1980;  39 348-57
  PubMedGoogle Scholar
• 3 Tie E G, Zeng L, Jin J, Xu B S, Sun Z W, Wang Y F. et al . The principle of herbals on drug abstinence.  Chin J Drug Depend. 1999;  8 305-9
  PubMedGoogle Scholar
• 4 Ramarao P, Bhargava H N. Antagonism of the acute pharmacological actions of morphine by Panax ginseng extract.  Gen Pharmacol. 1990;  21 877-80
  PubMedGoogle Scholar
• 5 Huong N T, Matsumoto K, Yamasaki K, Duc N M, Nham N T, Watanabe H. Majonoside-R2, a major constituent of Vietnamese ginseng, attenuates opioid-induced antinociception.  Pharmacol Biochem Behav. 1997;  57 285-91
  CrossrefPubMedGoogle Scholar
• 6 Li Z, Wu C F, Pei G, Guo Y Y, Li X. Antagonistic effect of pseudoginsengside-F11 on the behavioral actions of morphine in mice.  Pharmacol Biochem Behav. 2000;  66 595-601
  CrossrefPubMedGoogle Scholar
• 7 Kim H S, Jang C G, Oh K W, Oh S, Rheu H M, Rhee G S. et al . Effects of ginseng total saponin on morphine-induced hyperactivity and conditioned place preference in mice.  J Ethnopharmacol. 1998;  60 33-42
  CrossrefPubMedGoogle Scholar
• 8 Cha E Y, Mouledous L, Harris J R, Weech M A, Gutstein H B. Nitroglycerin inhibits the development of morphine tolerance and dependence in rats.  Pharmacol Biochem Behav. 2003;  74 551-7
  CrossrefPubMedGoogle Scholar
• 9 Choi S, Jung S Y, Rhim H, Jeong S W, Lee S M, Nah S Y. Evidence that ginsenosides prevent the development of opioid tolerance at the central nervous system.  Life Sci. 2000;  67 969-75
  CrossrefPubMedGoogle Scholar
• 10 Takahashi M, Tokuyama S, Kaneto H. Anti-stress effect of ginseng on the inhibition of the development of morphine tolerance in stressed mice.  Jpn J Pharmacol. 1992;  59 399-404
  PubMedGoogle Scholar
• 11 Bhargava H N, Ramarao P. The effect of Panax ginseng on the development of tolerance to the pharmacological actions of morphine in the rat.  Gen Pharmacol. 1991;  22 521-5
  PubMedGoogle Scholar
• 12 Kim H S, Hong Y T, Jang C G. Effects of the ginsenosides Rg1 and Rb1 on morphine-induced hyperactivity and reinforcement in mice.  J Pharm Pharmacol. 1998;  50 555-60
  PubMedGoogle Scholar

Dr. C. F. Wu

Department of Pharmacology

Shenyang Pharmaceutical University

Shenyang 110016

P. R. China

Phone: +86-24-2384-3567

Fax: +86-24-2384-3567

Email: wucf@syphu.edu.cn; wuchunf@21cn.com