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DOI: 10.1055/s-2004-819148
Effects of CD82-siRNA in thyroid carcinoma cell line FTC-133
RNA interference is a cellular process of gene silencing in which small duplexes of RNA specifically target a homologous sequence for cleavage by cellular ribonucleases. It has become a routine tool for transient knock down of gene expression in a wide range of organisms. In the present study, by using designed small interfering 21-mer RNAs (siRNA) directed against CD82, we investigated the protective effects of CD82 silencing on follicular thyroid carcinoma cell line 133. The expression of CD82 mRNA (RT-PCR) were examined at 24, 48, 72 hours and 1 week after siRNA-transfection. Additionally immunohistochemistry analysis was done. As negative control served a fluorescein labelled non-specific siRNA. At 24, 48 and 72 hours after transfection with CD82 siRNA CD82 expression is reduced in FTC-133 at both transcript and protein levels. Compared to untreated cells, the CD82 mRNA level and the CD82 protein level were decreased more than 70% (at the concentration of 2µg siRNA, p<0.05). Treatment of cells with synthetic siRNA was effective more than 72h. Our studies demonstrate that the siRNA directed against CD82 markedly decreased CD82 gene expression. SiRNA could target the specific factors whose expression is altered in malignancy and may have the potential as a therapeutic modality to treat human thyroid cancer.
Supported in part by the Pinguin-Stiftung