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Thromb Haemost 1993; 70(06): 1014-1018
DOI: 10.1055/s-0038-1649717
Original Article
Fibrinolysis
Schattauer GmbH Stuttgart

Frangulin B, an Antagonist of Collagen-Induced Platelet Aggregation and Adhesion, Isolated from Rhamnus formosana

Che-Ming Teng
1   The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei
,
Chien-Huang Lin
1   The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei
,
Chun-Nan Lin
2   Natural Products Research Center, Kaohsiung Medical College, Kaohsiung, Taiwan
,
Mei-lng Chung
2   Natural Products Research Center, Kaohsiung Medical College, Kaohsiung, Taiwan
,
Tur-Fu Huang
1   The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei
› Author Affiliations
Further Information

Publication History

Received 04 December 1992

Accepted after revision 12 July 1993

Publication Date:
06 July 2018 (online)

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Summary

Emodin and its glycoside frangulin B were isolated from the plant Rhamnus formosana. Emodin inhibited the aggregation of rabbit platelets induced by arachidonic acid and collagen, without affecting that by ADP or PAF, while emodin acetate had no any antiplatelet effect. Frangulin B inhibited selectively and concentration-dependently collagen-induced aggregation and ATP release in rabbit platelets, without affecting those induced by arachidonic acid, ADP, PAF and thrombin. Frangulin B also inhibited the platelet aggregation induced by trimucytin which was reported to be a collagen receptor agonist isolated from Trimeresurus muscrosquamatus snake venom. The aggregability of platelets inhibited by frangulin B could be recovered after washing the platelets. Frangulin B also selectively suppressed the thromboxane B2 formation caused by collagen, but not those by arachidonic acid and thrombin. Similarly, the formation of inositol phosphate caused by collagen was also suppressed by frangulin B, while that of PAF or thrombin was not affected. In the presence of PGE1, frangulin B also decreased Mg2+-dependent platelet adhesion to collagen. It is concluded that frangulin B may be an antagonist of collagen receptor in platelet membrane.

 

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