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Horm Metab Res 2023; 55(12): 876-884
DOI: 10.1055/a-2179-0283
Original Article: Endocrine Research

Hsa_circ_0080608 Attenuates Lung Cancer Progression by Functioning as a Competitive Endogenous RNA to Regulate the miR-661/ADRA1A Pathway

Chengbo Ren
1   Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
,
Ling Cui
1   Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
,
Ruibiao Li
1   Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
,
Xiao Song
1   Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
,
Jinqiu Li
1   Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
,
Qiang Xi
1   Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
,
Zhilin Zhang
1   Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
,
Lixia Zhao
2   Department of Internal Medicine Oncology, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China
› Author Affiliations Funding Information The 2022 Medical Science Research Project of Hebei Province — 20220618
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Abstract

Circular RNAs (circRNAs) participate in the progression of human cancers and have been broadly elucidated. Here, we aimed to elucidate the roles and functional mechanisms of hsa_circ_0080608 (circ_0080608) in lung cancer. Quantitative real-time PCR (qPCR) was performed to assess the mRNA expression levels of circ_0080608, miR-661, and adrenoceptor alpha 1A (ADRA1A). Western blotting was performed to measure ADRA1A protein levels. CCK-8, colony formation, and Transwell assays were performed to determine the effect of circ_0080608 on cell proliferation and migration. Animal models were used to assess how circ_0080608 influences tumor progression in vivo. The binding relationships of miR-661’s with circ_0080608 and ADRA1A was confirmed using dual-luciferase reporter and RIP assays. Circ_0080608 exhibited relatively low expression in lung cancer samples and cells. Lung cancer cells overexpressing circ_0080608 exhibited reduced migratory and proliferative abilities. Additionally, circ_0080608 binds to miR-661 and operates as a competing endogenous RNA (ceRNA) and shares a miR-661 binding site with the 3’ UTR of ADRA1A. Furthermore, circ_0080608 inversely regulates miR-661 expression, consequently restraining the aggressive behavior of lung cancer cells. Lung cancer cells overexpressing ADRA1A also exhibit repressed migratory and proliferative abilities. However, reintroduction of miR-661 led to a decline in ADRA1A expression, thereby attenuating the functional effects of ADRA1A. Circ_0080608 impedes lung cancer progression by regulating the miR-661/ADRA1A pathway. Our findings provide new insights into the progression of lung cancer.

Key words

lung cancer - circ_0080608 - miR-661 - ADRA1A


Publication History

Received: 06 May 2023

Accepted after revision: 11 September 2023

Article published online:
11 October 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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