Antimicrobial Activity of Seven Hypericum Entities from Central Italy

 

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Abstract

Methanol-acetone extracts from aerial parts of seven entities of the genus Hypericum [H. perforatum L. subsp. perforatum, H. perforatum L. subsp. veronense (Schrank) Ces., H. montanum L., H. hyssopifolium Chaix, H. hirsutum L., H. hircinum L. subsp. majus (Aiton) N. Robson, H. tetrapterum Fr.] growing in the Appennino Umbro-Marchigiano (central Italy) were tested in vitro for their antimicrobial activity against two Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecalis), two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and the yeast Candida albicans, by the Kirby-Bauer agar diffusion method. The two H. perforatum subspecies were particularly active against Gram-positive and Gram-negative bacteria, supporting the use of these plants in the traditional medicine of central Italy to treat wounds, skin and infectious diseases. However significant values of inhibition were given also by H. hirsutum against S. aureus and P. aeruginosa, and by H. hyssopifolium, H. hircinum subsp. majus and H. tetrapterum against C. albicans. These results suggest that hypericin and hyperforin are the main components responsible for antimicrobial activity, but not the only ones, as other extracts, showing a low level of these compounds, possess evident activity. Isolation of pure compounds from the most active extracts is in progress.

References

• 1 Pieroni A, Quave C L, Villanelli M L, Mangino P, Sabbatini G, Santini L. et al . Ethnopharmacognostic survey on the natural ingredients used in folk cosmetics, cosmeceuticals and remedies for healing skin diseases in the inland Marches, Central-Eastern Italy.  J Ethnopharmacol. 2004;  91 331-44.
  CrossrefPubMedGoogle Scholar
• 2 Guarrera P M. Traditional phytotherapy in central Italy (Marche, Abruzzo, and Latium).  Fitoterapia. 2005;  76 1-25.
  CrossrefPubMedGoogle Scholar
• 3 Pieroni A, Quave C L, Santoro R F. Folk pharmaceutical knowledge in the territory of the Dolomiti Lucane, inland southern Italy.  J Ethnopharmacol. 2004;  95 373-84.
  CrossrefPubMedGoogle Scholar
• 4 Bauer A W, Kirby W MM, Sherris J C, Truck M. Antibiotic susceptibility testing by a standardized single disc method.  Am J Clin Pathol. 1966;  45 493-6.
  PubMedGoogle Scholar
• 5 Avato P, Raffo F, Guglielmi G, Vitali C, Rosato A. Extracts from St John’s wort and their antimicrobial activity.  Phytother Res. 2004;  18 230-2.
  CrossrefPubMedGoogle Scholar
• 6 Conforti F, Statti G A, Tundis R, Bianchi A, Agrimonti C, Sacchetti G. et al . Comparative chemical composition and variability of biological activity of methanolic extracts from Hypericum perforatum L.  Nat Prod Res. 2005;  19 295-303.
  CrossrefPubMedGoogle Scholar
• 7 Maleš Ž, Brantner A H, Sović K, Pilepić K H, Plazibat M. Comparative phytochemical and antimicrobial investigations of Hypericum perforatum L. subsp. perforatum and H. perforatum subsp. angustifolium (DC.) Gaudin.  Acta Pharm. 2006;  56 359-67.
  PubMedGoogle Scholar
• 8 Pistelli L, Bertoli A, Zucconelli S, Morelli I, Panizzi L, Menichini F. Antimicrobial activity of crude extracts and pure compounds of Hypericum hircinum .  Fitoterapia. 2000;  71 138-40.
  CrossrefPubMedGoogle Scholar
• 9 Gibbons S, Ohlendorf B, Johnsen I. The genus Hypericum - a valuable resource of anti-staphylococcal leads.  Fitoterapia. 2002;  73 300-4.
  CrossrefPubMedGoogle Scholar
• 10 Toker Z, Kizil G, Çetin Özen H, Kizil M, Ertekin S. Compositions and antimicrobial activities of the essential oils of two Hypericum species from Turkey.  Fitoterapia. 2006;  77 57-60.
  CrossrefPubMedGoogle Scholar
• 11 Haslam E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action.  J Nat Prod. 1996;  59 205-15.
  CrossrefPubMedGoogle Scholar
• 12 Dolara P, Luceri C, De Filippo C, Femia A P, Giovannelli L, Caderni G. et al . Red wine polyphenols influence carcinogenesis, intestinal microflora, oxidative damage and gene expression profiles of colonic mucosa in F344 rats.  Mutat Res. 2005;  591 237-46.
  PubMedGoogle Scholar
• 13 Rios J L, Recio M C, Villar A. Screening methods for natural products with antimicrobial activity: a review of the literature.  J Ethnopharmacol. 1988;  23 127-49.
  PubMedGoogle Scholar
• 14 Maggi F, Ferretti G, Pocceschi N, Menghini L, Ricciutelli M. Morphological, histochemical and phytochemical investigation of the genus Hypericum of the Central Italy.  Fitoterapia. 2004;  75 702-11.
  CrossrefPubMedGoogle Scholar
• 15 Brolis M, Gavetta B, Fuzzati N, Pace R, Panzeri F, Peterlongo F. Identification by high-performance liquid chromatography-diode array detection-mass spectrometry and quantification by highperformance liquid chromatography-UV absorbance detection of active constituents of Hypericum perforatum .  J Chromatogr A. 1998;  825 9-16.
  CrossrefPubMedGoogle Scholar
• 16 Arnott C. Extraction and HPLC of Hypericin, article available online at http://www.sbc.edu/honors/HJSpr03/Arnott.htm. 2003.
  Google Scholar

Dr. F. Maggi

University of Camerino

Faculty of Pharmacy

Department of Environmental Sciences

Section of Botany and Ecology

via Pontoni 5

62032 Camerino

Italy

Phone: +39-0737-404-506

Fax: +39-0737-404-508

Email: filippo.maggi@unicam.it

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