Basic–alimentary tractStomach Development Is Dependent on Fibroblast Growth Factor 10/Fibroblast Growth Factor Receptor 2b–Mediated Signaling
Section snippets
Generation of Mice
All animal experiments were performed according to UK Home Office regulations. The targeted disruption of Fgfr2b19 and Fgf1023 has been described previously. Both Fgfr2b and Fgf10 heterozygotes were intercrossed to generate homozygous null embryos at the required embryonic stage, and yolk sacs were used for genotyping by polymerase chain reaction.19 The morning of vaginal plug appearance was considered E0.5. Control mice consisted of wild-type (wt) and heterozygous littermates, the latter being
Absence of Fgfr2b or Its Ligand Fgf10 Results in Defective Stomach Growth and Patterning During Development
Stomachs were dissected from wt, Fgfr2b−/−, and Fgf10−/− mice between E16.5 and E18.5. Mutant stomachs were small, although roughly in proportion to the reduced size of the mouse as a whole (Figure 1 A). In both cases, the forestomach appeared to show a slight loss of definition of the longitudinal folds but no other gross changes. The muscular wall of the stomach appeared normal. The glandular epithelial lining of the corpus region of both Fgfr2b−/− and Fgf10−/− mice appeared devoid of normal
Discussion
We report the gastric developmental defects exhibited by mice null for Fgf10 or its receptor, Fgfr2b, during fetal development. Mutant stomachs from both mutants were reduced in size when compared with those from normal littermates. This finding is consistent with an overall proportionate reduction in size of mutants versus controls and may also be due to a decrease in proliferation observed here at E13.5.19, 23, 24 Histologic analysis showed that the glandular epithelial architecture was
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