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Molecular characterization and genetic relationships among most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa (Sapotaceae) using RAPD and SSR markers

Caractérisation moléculaire et relation génétique entre les morphotypes les plus connus d’une espèce marocaine rare et menacée, Argania spinosa (Sapotaceae) à l’aide de marqueurs RAPD et SSR

Annals of Forest Science volume 65, page 805 (2008)Cite this article

Abstract

  • • The objective of this work is the molecular characterization of most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa.

  • • Eighteen RAPD markers and twenty SSR markers have been assayed in 38 argan tree accessions from the three most commonly identified morphotypes: oval, spherical and spindle fruit types.

  • • A total of 140 polymorphic RAPD bands were detected out of 146 bands. The number of presumed alleles revealed by the SSR analysis ranged from 1 to 5 alleles per locus with a total number of 32 alleles identified. Results demonstrated an extensive genetic variability within the tested argan accessions. RAPDs presented a high level of polymorphism and greater information content than SSRs.

  • • Our results could indicate that Vitellaria paradoxa is genetically closer to argan than Manilkara huberi. In addition, the correlation between the clustering based on RAPD and SSR markers were in general low. The observed clustering could be better explained according to geographic proximity than morphotype. For this reason the traditional morphological characterization of the argan accessions in morphotypes (according to fruit phenotype) seem not to be in correlation to the real genetic background (genotype) of this specie. The implications of these results in the creation of effective germplasm core collection in argan have been also discussed.

Résumé

  • • L’objectif de ce travail consiste en la caractérisation moléculaire et l’étude des relations génétiques entre des morphotypes identifiés chez une espèce marocaine rare et menacée, l’arganier (Argania spinosa).

  • • Pour cela, dix-neuf marqueurs RAPD et vingt marqueurs SSR on été testés dans 38 accessions d’arganier des trois morphotypes du fruit identifiés : ovale, sphérique et broche.

  • • En utilisant la technique d’amplification au hasard de l’ADN (RAPD), 140 fragments polymorphes ont été détectés parmi les 146 amplifiés. Le nombre d’allèle révélé par l’analyse des marqueurs microsatelittes (SSRs) varie de un à cinq allèles par locus avec un total de 32 allèles identifiés. Les résultats démontrent une grande variabilité génétique au sein des accessions d’arganier testées. Les marqueurs RAPD présentent un taux de polymorphisme et une hétérozygotie attendue supérieurs aux SSR.

  • • Les résultats indiquent également que Vitellaria paradoxa est plus proche génétiquement de l’arganier que Manilkara huberi. La corrélation entre les groupements observés dépend plus de la proximité géographique que de la classification selon la forme du fruit (morphotypes). La classification traditionnelle par morphotypes n’est donc pas en accord avec l’analyse génotypique chez cette espèce. L’apport de ces résultats pour la création d’une collection de référence chez cette espèce est discuté.

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Authors and Affiliations

  1. Faculté des Sciences Semlalia, University Cadi-Ayyad of Marrakech, Morocco

    Khalid Majourhat, Youssef Jabbar & Abdellatif Hafidi

  2. Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo (Murcia), Spain

    Khalid Majourhat & Pedro Martínez-Gómez

Authors
  1. Khalid Majourhat
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  2. Youssef Jabbar
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  3. Abdellatif Hafidi
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  4. Pedro Martínez-Gómez
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Correspondence to Pedro Martínez-Gómez.

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Majourhat, K., Jabbar, Y., Hafidi, A. et al. Molecular characterization and genetic relationships among most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa (Sapotaceae) using RAPD and SSR markers. Ann. For. Sci. 65, 805 (2008). https://doi.org/10.1051/forest:2008069

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Annals of Forest Science

ISSN: 1297-966X