FISH and chips: chromosomal analysis on microfluidic platforms
FISH and chips: chromosomal analysis on microfluidic platforms
- Author(s): V.J. Sieben ; C.S. Debes Marun ; P.M. Pilarski ; G.V. Kaigala ; L.M. Pilarski ; C.J. Backhouse
- DOI: 10.1049/iet-nbt:20060021
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- Author(s): V.J. Sieben 1 ; C.S. Debes Marun 2 ; P.M. Pilarski 1 ; G.V. Kaigala 1 ; L.M. Pilarski 2 ; C.J. Backhouse 1
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View affiliations
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Affiliations:
1: Department of Electrical and computer, Engineering, University of Alberta, Canada
2: Department of Electrical and computer, Engineering, Cross Cancer Institute, Edmonton, Canada
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Affiliations:
1: Department of Electrical and computer, Engineering, University of Alberta, Canada
- Source:
Volume 1, Issue 3,
June 2007,
p.
27 – 35
DOI: 10.1049/iet-nbt:20060021 , Print ISSN 1751-8741, Online ISSN 1751-875X
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Interphase fluorescence in situ hybridisation (FISH) is a sensitive diagnostic tool used for the detection of alterations in the genome on cell-by-cell basis. However, the cost-per-test and the technical complexity of current FISH protocols have slowed its widespread utilisation in clinical settings. For many cancers, the lack of a cost-effective and informative diagnostic method has compromised the quality of life for patients. We present the first demonstration of a microchip-based FISH protocol, coupled with a novel method to immobilise peripheral blood mononuclear cells inside microfluidic channels. These first on-chip implementations of FISH allow several chromosomal abnormalities associated with multiple myeloma to be detected with a ten-fold higher throughput and 1/10‐th the reagent consumption of the traditional slide-based method. Moreover, the chip test is performed within hours whereas the conventional protocol required days. In addition, two on-chip methods to enhance the hybridisation aspects of FISH have been examined: mechanical and electrokinetic pumping. Similar agitation methods have led to significant improvements in hybridisation efficiency with DNA microarray work, but with this cell-based method the benefits were moderate. On-chip FISH technology holds promise for sophisticated and cost-effective screening of cancer patients at every clinic visit.
Inspec keywords: cellular biophysics; fluorescence; microfluidics; lab-on-a-chip; cancer; patient diagnosis
Other keywords:
Subjects: Micromechanical and nanomechanical devices and systems; Microsensors and nanosensors; Biomedical measurement and imaging; Cellular biophysics; Chemical sensors; Patient diagnostic methods and instrumentation; Chemical variables measurement
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