Issue 2, 2024

Selective FRET nano probe based on carbon dots and naphthalimide–isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury

Abstract

Drug-induced liver injury (DILI) is the most common cause for acute liver failure in the USA and Europe. However, most of DILI cases can recover or be prevented if treatment by the offending drug is discontinued. Recent research indicates that peroxynitrite (ONOO) can be a potential indicator to diagnose DILI at an early stage. Therefore, the establishment of an assay to detect and track ONOO in DILI cases is urgently needed. Here, a FRET-based ratiometric nano fluorescent probe CD–N-I was developed to detect ONOO with high selectivity and excellent sensitivity. This probe consists of carbon dots and a naphthalimide–isatin peroxynitrite sensing system assembled based on electrostatic interactions. Using CD–N-I we were able to detect exogenous ONOO in live cells and endogenous ONOO in APAP-induced liver injury of HepG2 cells.

Graphical abstract: Selective FRET nano probe based on carbon dots and naphthalimide–isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury

Supplementary files

Article information

Article type
Edge Article
Submitted
23 Sep 2023
Accepted
22 Nov 2023
First published
14 Dec 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2024,15, 757-764

Selective FRET nano probe based on carbon dots and naphthalimide–isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury

Y. Wu, L. Sun, H. Han, X. He, W. Cao and T. D. James, Chem. Sci., 2024, 15, 757 DOI: 10.1039/D3SC05010F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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