Issue 2, 2024

Multiplexed immunosensing of cancer biomarkers on a split-float-gate graphene transistor microfluidic biochip

Abstract

This work reports the development of a novel microfluidic biosensor using a graphene field-effect transistor (GFET) design for the parallel label-free analysis of multiple biomarkers. Overcoming the persistent challenge of constructing μm2-sized FET sensitive interfaces that incorporate multiple receptors, we implement a split-float-gate structure that enables the manipulation of multiplexed biochemical functionalization using microfluidic channels. Immunoaffinity biosensing experiments are conducted using the mixture samples containing three liver cancer biomarkers, carcinoembryonic antigen (CEA), α-fetoprotein (AFP), and parathyroid hormone (PTH). The results demonstrate the capability of our label-free biochip to quantitatively detect multiple target biomarkers simultaneously by observing the kinetics in 10 minutes, with the detection limit levels in the nanomolar range. This microfluidic biosensor provides a valuable analytical tool for rapid multi-target biosensing, which can be potentially utilized for domiciliary tests of cancer screening and prognosis, obviating the need for sophisticated instruments and professional operations in hospitals.

Graphical abstract: Multiplexed immunosensing of cancer biomarkers on a split-float-gate graphene transistor microfluidic biochip

Supplementary files

Article information

Article type
Paper
Submitted
18 Aug 2023
Accepted
03 Dec 2023
First published
05 Dec 2023

Lab Chip, 2024,24, 317-326

Multiplexed immunosensing of cancer biomarkers on a split-float-gate graphene transistor microfluidic biochip

C. Wang, T. Wang, Y. Gao, Q. Tao, W. Ye, Y. Jia, X. Zhao, B. Zhang and Z. Zhang, Lab Chip, 2024, 24, 317 DOI: 10.1039/D3LC00709J

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