Mass filtering combined with photochemical derivatization enables high throughput mass spectrometric analysis of unsaturated phosphatidylcholine isomers

Huimin Li; Qian Xiong; Hao Wu; Yunmei Zhang; Ke Zhuang; Yan Zhao; Hong Zhang; Lunzhao Yi

doi:10.1039/D3AY01829F

Mass filtering combined with photochemical derivatization enables high throughput mass spectrometric analysis of unsaturated phosphatidylcholine isomers†

Huimin Li,^a Qian Xiong,^a Hao Wu,^bc Yunmei Zhang,^c Ke Zhuang,^c Yan Zhao,^cd Hong Zhang*^cd and Lunzhao Yi

*^ab

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* Corresponding authors

^a Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, China
E-mail: yilunzhao@kust.edu.cn

^b Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, China

^c Department of Cardiology, First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China
E-mail: zhkhzx@yeah.net

^d College of Medicine, Kunming University of Science and Technology, Kunming, China

Abstract

Phosphatidylcholines (PCs) are closely related to coronary heart disease, such as myocardial infarction. The analysis of the deep structure of PCs is of great significance for exploring the effects of exercise rehabilitation and lipid metabolism. Here, we present a mass filtering combined with photochemical derivatization method for rapid screening and accurate identification of the C [double bond, length as m-dash] C position and sn-location isomer of PCs. This method is simple to execute and easily implementable for routine analysis. The accurate qualitative and quantitative analysis of PCs and isomers facilitates the discovery of biomarkers for exercise rehabilitation of patients with myocardial infarction.

This article is part of the themed collection: Analytical Methods HOT Articles 2023

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DOI: https://doi.org/10.1039/D3AY01829F
Article type: Communication
Submitted: 15 Oct 2023
Accepted: 06 Nov 2023
First published: 07 Nov 2023

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Anal. Methods, 2024,16, 371-377

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Mass filtering combined with photochemical derivatization enables high throughput mass spectrometric analysis of unsaturated phosphatidylcholine isomers

H. Li, Q. Xiong, H. Wu, Y. Zhang, K. Zhuang, Y. Zhao, H. Zhang and L. Yi, Anal. Methods, 2024, 16, 371 DOI: 10.1039/D3AY01829F

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Analytical Methods

Mass filtering combined with photochemical derivatization enables high throughput mass spectrometric analysis of unsaturated phosphatidylcholine isomers†

Abstract

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Mass filtering combined with photochemical derivatization enables high throughput mass spectrometric analysis of unsaturated phosphatidylcholine isomers

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