Issue 11, 2022
From the journal:

Organic Chemistry Frontiers

Discovery and characterization of a novel sub-group of UbiA-type terpene cyclases with a distinct motif I†

Pan Luo,‡a   Jian-Ming Lv,‡a   Yan-Feng Xie,a   Liang-Yan Xiao,a   Sheng-Ying Qin,b   Guo-Dong Chen,a   Xiao-Zhou Luo,c   Dan Hu ORCID logo *ac  and  Hao Gao ORCID logo *a  

* Corresponding authors

a Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Jinan University, Guangzhou 510632, China
E-mail: tghao@jnu.edu.cn, thudan@jnu.edu.cn

b Clinical Experimental Center, First Affiliated Hospital of Jinan University, Guangzhou 510630, China

c Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

Abstract

A novel sub-group of UbiA-type terpene cyclases was identified from fungi, which contain a distinct motif I NQXXXXXXXED instead of the known NXXX(G/A)XXXD found in all reported UbiA-type terpene cyclases. Subsequent genome mining revealed the prevalence of this novel sub-group of UbiA-type terpene cyclases in fungi. Functional analysis of the representative cyclases indicated that they are able to generate diverse sesquiterpenes via a bisabolyl cation intermediate. Moreover, unlike canonical classes I and II terpene cyclases, these enzymes can generate the same products even if they are phylogenetically separated, and produce distinct products even if they are phylogenetically close, suggesting that it is difficult to predict their functions based on sequence similarity.

Graphical abstract: Discovery and characterization of a novel sub-group of UbiA-type terpene cyclases with a distinct motif I

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Article information

DOI
https://doi.org/10.1039/D2QO00408A
Article type
Research Article
Submitted
12 Mar 2022
Accepted
19 Apr 2022
First published
21 Apr 2022

Org. Chem. Front., 2022,9, 3057-3060

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Discovery and characterization of a novel sub-group of UbiA-type terpene cyclases with a distinct motif I

P. Luo, J. Lv, Y. Xie, L. Xiao, S. Qin, G. Chen, X. Luo, D. Hu and H. Gao, Org. Chem. Front., 2022, 9, 3057 DOI: 10.1039/D2QO00408A

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