A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa

Xiaoliang Li; Sixian Wu; Kangkang Yu; Jianwen Hou; Chuan Jiang; Kun Li; Zhiwen Chu; Xiaohui Jiang; Xiaoqi Yu; Wenming Xu

doi:10.1039/D1TB00108F

A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa†

Xiaoliang Li,

‡^a Sixian Wu,‡^a Kangkang Yu,^b Jianwen Hou,^a Chuan Jiang,^a Kun Li,

^c Zhiwen Chu,^d Xiaohui Jiang,^e Xiaoqi Yu

*^c and Wenming Xu*^af

Author affiliations

* Corresponding authors

^a Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine, SCU-CUHK, Key Laboratory of Obstetric, Gynaecologic and Paediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China
E-mail: xuwenming@scu.edu.cn

^b Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, China

^c Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu, China
E-mail: xqyu@scu.edu.cn

^d West China School of Pharmacy, Sichuan University, Chengdu, China

^e Human Sperm Bank, West China Second University Hospital, Sichuan University, Chengdu, China

^f Reproductive Endocrinology and Regulation Laboratory, West China Second University Hospital, Sichuan University, Chengdu, China

Abstract

A dual-site controlled pH probe, which is composed of gold nanoparticles and modified with rhodamine and fluorescein derivatives, was applied to sensitively monitor intracellular pH changes in sperm. The pH probe revealed the intracellular pH of sperm under different conditions and demonstrated the lower pH in asthenozoospermia patients as compared to healthy individuals. Importantly, the pH probe can help screen for healthy sperm.

Journal of Materials Chemistry B

A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa†

Abstract

Supplementary files

Article information

Download Citation

Permissions

A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa

Social activity

Search articles by author

Spotlight

Advertisements