Rotational scan digital LAMP for accurate quantitation of nucleic acids

Mengcheng Jiang; Peiyu Liao; Yue Sun; Xinyang Shao; Zitian Chen; Peng Fei; Jianbin Wang; Yanyi Huang

doi:10.1039/D1LC00114K

Rotational scan digital LAMP for accurate quantitation of nucleic acids†

Mengcheng Jiang,^ab Peiyu Liao,^ab Yue Sun,^bc Xinyang Shao,^bc Zitian Chen,^ab Peng Fei,

^d Jianbin Wang^e and Yanyi Huang

*^abcfg

Author affiliations

* Corresponding authors

^a Materials Science and Engineering, College of Engineering, Peking University, Beijing, China
E-mail: yanyi@pku.edu.cn

^b Biomedical Pioneering Innovation Center (BIOPIC) and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China

^c Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China

^d School of Optical and Electronic Information-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China

^e School of Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing, China

^f College of Chemistry and Molecular Engineering, and, Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China

^g Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, China

Abstract

Digital quantitation of nucleic acids is precise and sensitive because of its molecular-level resolution. However, only several quantitation formats are common, especially pertaining to how one obtains digital signals from multiple droplets. Here we present rotational scan digital loop-mediated amplification, termed RS-dLAMP. Droplets generated by centrifugation undergo isothermal loop-mediated amplification (LAMP), and self-tile by gravitation into a tubular space between two coaxial cylinders, which are then rotated and scanned to acquire droplet fluorescence signals. RS-dLAMP is quantitatively comparable to commercial digital PCR, yet has higher throughput. Moreover, by sealing the sample throughout analysis, RS-dLAMP eliminates contamination, facilitating point-of-care diagnosis and other applications.

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DOI: https://doi.org/10.1039/D1LC00114K
Article type: Paper
Submitted: 14 Feb 2021
Accepted: 19 Apr 2021
First published: 20 Apr 2021

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Lab Chip, 2021,21, 2265-2271

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Rotational scan digital LAMP for accurate quantitation of nucleic acids

M. Jiang, P. Liao, Y. Sun, X. Shao, Z. Chen, P. Fei, J. Wang and Y. Huang, Lab Chip, 2021, 21, 2265 DOI: 10.1039/D1LC00114K

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Lab on a Chip

Rotational scan digital LAMP for accurate quantitation of nucleic acids†

Abstract

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Rotational scan digital LAMP for accurate quantitation of nucleic acids

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