Organoarsenic probes to study proteins by NMR spectroscopy

Mithun C. Mahawaththa; Henry W. Orton; Ibidolapo Adekoya; Thomas Huber; Gottfried Otting; Christoph Nitsche

doi:10.1039/D1CC06497E

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Organoarsenic probes to study proteins by NMR spectroscopy†

Mithun C. Mahawaththa,‡^a Henry W. Orton,‡^b Ibidolapo Adekoya,^a Thomas Huber,^a Gottfried Otting

*^b and Christoph Nitsche

*^a

Author affiliations

* Corresponding authors

^a Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
E-mail: christoph.nitsche@anu.edu.au

^b ARC Centre of Excellence for Innovations in Peptide and Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
E-mail: gottfried.otting@anu.edu.au

Abstract

Arsenical probes enable structural studies of proteins. We report the first organoarsenic probes for nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy to study proteins in solutions. These probes can be attached to irregular loop regions. A lanthanide-binding tag induces sizable pseudocontact shifts in protein NMR spectra of a magnitude never observed for small paramagnetic probes before.

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Article information

DOI: https://doi.org/10.1039/D1CC06497E
Article type: Communication
Submitted: 18 Nov 2021
Accepted: 15 Dec 2021
First published: 15 Dec 2021

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Chem. Commun., 2022,58, 701-704

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Organoarsenic probes to study proteins by NMR spectroscopy

M. C. Mahawaththa, H. W. Orton, I. Adekoya, T. Huber, G. Otting and C. Nitsche, Chem. Commun., 2022, 58, 701 DOI: 10.1039/D1CC06497E

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Chemical Communications

Organoarsenic probes to study proteins by NMR spectroscopy†

Abstract

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Organoarsenic probes to study proteins by NMR spectroscopy

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